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Transcriptome-wide analysis of N6-methyladenosine uncovers its regulatory role in gene expression in the lepidopteran Bombyx mori.
Insect Molecular Biology ( IF 2.6 ) Pub Date : 2019-04-29 , DOI: 10.1111/imb.12584
B Li 1, 2 , X Wang 1, 2 , Z Li 1, 3 , C Lu 1 , Q Zhang 1 , L Chang 1 , W Li 1 , T Cheng 2 , Q Xia 1, 3 , P Zhao 1, 3
Affiliation  

N6‐methyladenosine (m6A) methylation is the most common form of RNA modification in eukaryotes and has been shown to act as an important epitranscriptomic marker, which can regulate gene expression, messenger RNA stability, alternative splicing and translation efficiency. Recent progress on determining insect m6A function has been limited to the dipteran Drosophila melanogaster, in which m6A is involved in neuronal functions and sex determination. Methylation and function of m6A in other insects, however, remain unknown. Here, we investigated a transcriptome‐wide profile of m6A in the lepidopteran Bombyx mori and identified the methyltransferase subunits B. mori methyltransferase‐like 3 (BmMETTL3) and BmMETTL14 in the m6A methylation pathway. Strikingly, loss of BmMETTL3 and BmMETTL14 in cultured Bmori cells led to arrest of cell cycle progression and caused deficiency of chromosome alignment and segregation. Specifically, we identified 2853 m6A peaks representing transcripts of 2043 genes, and the target genes with m6A methylation were shown to be involved in gene expression and translation. It was interesting that we found that the highly expressed genes tended to be methylated by m6A, and comparative analysis of RNA m6A and DNA N6‐methyladenine (6mA) revealed two distinct regulatory mechanisms for gene expression. Overall, our work suggests RNA m6A and DNA 6mA play important roles in RNA and DNA epigenetic regulation in Bmori.

中文翻译:

N6-甲基腺苷的全转录组分析揭示了其在鳞翅目家蚕基因表达中的调节作用。

N6-甲基腺苷 (m6A) 甲基化是真核生物中最常见的 RNA 修饰形式,已被证明是一种重要的表观转录组标记,可以调节基因表达、信使 RNA 稳定性、选择性剪接和翻译效率。确定昆虫 m6A 功能的最新进展仅限于双翅目Drosophila melanogaster,其中 m6A 参与神经元功能和性别决定。然而,其他昆虫中 m6A 的甲基化和功能仍然未知。在这里,我们调查M6A在鳞翅目一个全基因组范围的个人资料家蚕,并确定了甲基亚单位家蚕m6A 甲基化途径中的甲基转移酶样 3 (BmMETTL3) 和 BmMETTL14。引人注目的是,培养B中 BmMETTL3 和 BmMETTL14 的丢失。 mori细胞导致细胞周期进程停滞并导致染色体排列和分离不足。具体而言,我们确定了代表 2043 个基因转录本的 2853 个 m6A 峰,并且显示具有 m6A 甲基化的靶基因参与基因表达和翻译。有趣的是,我们发现高表达的基因倾向于被 m6A 甲基化,对 RNA m6A 和 DNA N6-甲基腺嘌呤 (6mA) 的比较分析揭示了两种不同的基因表达调控机制。总的来说,我们的工作表明 RNA m6A 和 DNA 6mA 在B 的RNA 和 DNA 表观遗传调控中发挥重要作用
更新日期:2019-04-29
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