Background: Interest in the antioxidant and antidiabetic activity of natural products are growing vastly in the modern world. Thin layer chromatography-bioautography-mass spectroscopy (TLC-bioautography-MS) plays an important role in chemico-biological screening of natural sources. TLC combined with 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical, α-amylase and α-glucosidase bioassay were used to evaluate antioxidant and antidiabetic activities, respectively, in different extracts of Citrullus colocynthis (Hanzal), a well-known traditional Indian Unani medicinal plant. Objective: To develop a TLC-bioautographic-MS method for DPPH, α-amylase, and glucosidase inhibitors in different extract of C. colocynthis fruits. Method: Fruits of C. colocynthis were successively extracted with toluene, dichloromethane, ethyl acetate, methanol, and water. TLC solvents were developed, and bioautographic-MS analysis was carried out to identify the antioxidant and antidiabetic compounds. Results: HPTLC fingerprinting analysis showed maximum numbers of band separated in dichloromethane and ethyl acetate extracts of C. colocynthis, fourteen and thirteen at 254 and 366 nm, respectively. Whereas six and five separated bands were observed in toluene extract at 254 and 366 nm, respectively showed minimum numbers of metabolites. Based on TLC-bioautography-MS, maximum number of antioxidant compounds were identified in dichloromethane extract. Except aqueous extract of C. colocynthis, all the extracts have shown antidiabetic activity. On the other hand, there were no antioxidant compounds in methanolic extract of C. colocynthis. Conclusions: The results of this study reveal that TLC-bioautography-MS–guided strategy used to identify antioxidant and antidiabetic compounds of C. colocynthis is very useful technique for high-throughput screening of bioactive compounds. Highlights: TLC-MS bioautography is a simple and fast to enables bioactive compounds present in extracts.

中文翻译：

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高通量筛选和鉴定来自传统Unani药用植物Citrullus colocynthis Schrad的自由基清除和抗糖尿病化合物的TLC生物自动评估。

背景：在现代世界中，人们对天然产物的抗氧化和抗糖尿病活性的兴趣正在迅速增长。薄层色谱-生物自显影-质谱（TLC-生物自显影-MS）在天然来源的化学生物学筛选中起着重要作用。TLC结合2,2-二苯基-1-吡啶并肼基（DPPH）自由基，α-淀粉酶和α-葡萄糖苷酶生物测定法分别评估了香瓜（Chantrullus colocynthis）（汉萨尔）的不同提取物中的抗氧化和抗糖尿病活性。已知的传统印度Unani药用植物。目的：建立一种薄层色谱生物自动质谱法测定不同提取物中DPPH，α-淀粉酶和葡糖苷酶抑制剂的方法。C. colocynthis果实。方法：先后用甲苯，二氯甲烷，乙酸乙酯，甲醇和水提取co树的果实。开发了TLC溶剂，并进行了生物自传质谱分析以鉴定抗氧化剂和抗糖尿病化合物。结果： HPTLC指纹图谱分析显示co鱼的二氯甲烷和乙酸乙酯提取物中的最大条带分离数分别在254和366 nm处分别为14和13。而在甲苯提取物中分别在254和366 nm处观察到六个和五个分离的谱带，分别显示出最小的代谢物数量。基于TLC-生物自显影-MS，在二氯甲烷提取物中鉴定出最大数量的抗氧化剂化合物。除co鱼的水提取物外，所有提取物均显示出抗糖尿病活性。另一方面，C。colocynthis的甲醇提取物中没有抗氧化剂。结论：这项研究的结果表明，TLC-生物自传图谱-MS指导的策略可用于识别co鱼梭菌的抗氧化剂和抗糖尿病化合物，这对于高通量筛选生物活性化合物是非常有用的技术。亮点： TLC-MS生物自显影技术既简单又快速，可以使提取物中存在生物活性化合物。