Cryogenic transmission electron microscopy of high‐pressure freezing (HPF) samples is a well‐established technique for the analysis of liquid containing specimens. This technique enables observation without removing water or other volatile components. The HPF technique is less used in scanning electron microscopy (SEM) due to the lack of a suitable HPF specimen carrier adapter. The traditional SEM cryotransfer system (PP3000T Quorum Laughton, East Sussex, UK; Alto Gatan, Pleasanton, CA, USA) usually uses nitrogen slush. Unfortunately, and unlike HPF, nitrogen slush produces water crystal artefacts.

中文翻译：

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一种新的 HPF 标本载体适配器，用于使用冷冻扫描电子显微镜进行高压冷冻：两种应用：羟丙基甲基纤维素基质中的硬脂酸组织和小鼠心肌

高压冷冻 (HPF) 样品的低温透射电子显微镜是一种用于分析含有液体的样品的成熟技术。这种技术可以在不去除水或其他挥发性成分的情况下进行观察。由于缺乏合适的 HPF 样品载体适配器，HPF 技术在扫描电子显微镜 (SEM) 中使用较少。传统的 SEM 低温转移系统（PP3000T Quorum Laughton，East Sussex，UK；Alto Gatan，Pleasanton，CA，USA）通常使用氮泥。不幸的是，与 HPF 不同的是，氮泥会产生水晶体假象。