BACKGROUND AND AIM Inflammatory and methylation imbalances occur in patients with type 2 diabetes mellitus (T2DM). The aim of the present study was to analyze the effect of acute resistance exercise on the inflammatory profile and on DNA methylation of elderly patients with T2DM using metformin. METHODS For this purpose, we enrolled 22 male and female older adults (68.2 ± 5.3 years), of whom 13 had controlled T2DM (D) under metformin use and 9 were nondiabetics (ND). All subjects underwent a neuromuscular circuit (8 exercises in 40 min, with each exercise performed in 3 sets of 40 s each and a 20-s interval between repetitions). RESULTS The main results indicated a significant difference between groups for baseline interleukin (IL)-10, with a higher concentration in the D group compared to the ND group (p = 0.019). An increase in IL-6 concentration after intervention was observed in group D (p = 0.035). No effect was observed in total DNA methylation within or between groups. CONCLUSIONS The resistance training protocol applied in this study modulates the IL-10 and IL-6 concentrations in elderly people with T2DM and under metformin use, possibly as a result of physiological adaptations, with no effect on nondiabetic elderly. No effects on absolute levels of DNA methylation were observed.

中文翻译：

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阻力训练使用二甲双胍调节糖尿病老年人的体液性炎症（但不是DNA甲基化）。

背景与目的2型糖尿病（T2DM）患者发生炎症和甲基化失衡。本研究的目的是使用二甲双胍分析急性抵抗运动对老年T2DM患者的炎症和DNA甲基化的影响。方法为此，我们招募了22名男性和女性老年人（68.2±5.3岁），其中13名在使用二甲双胍的情况下控制了T2DM（D），而9名非糖尿病（ND）。所有受试者均进行了神经肌肉循环运动（40分钟内进行8次运动，每组运动进行3组，每组40 s，重复之间的间隔为20 s）。结果主要结果表明基线白细胞介素（IL）-10组之间存在显着差异，D组的浓度高于ND组（p = 0.019）。D组观察到干预后IL-6浓度增加（p = 0.035）。在组内或组之间未观察到总DNA甲基化的影响。结论本研究中使用的抗性训练方案可调节患有T2DM且使用二甲双胍的老年人的IL-10和IL-6浓度，这可能是由于生理适应所致，对非糖尿病老年人没有影响。没有观察到对DNA甲基化绝对水平的影响。对非糖尿病老人没有影响。没有观察到对DNA甲基化绝对水平的影响。对非糖尿病老人没有影响。没有观察到对DNA甲基化绝对水平的影响。