The way that chromatin is organized in three-dimensional nuclear space is now acknowledged as a factor critical for the major cell processes, like transcription, replication and cell division. Researchers have been armed with new molecular and imaging technologies to study this structure-to-function link of genomes, spearheaded by the introduction of the 'chromosome conformation capture' technology more than a decade ago. However, this technology is not without shortcomings, and novel variants and orthogonal approaches are being developed to overcome these. As a result, the field of nuclear organization is constantly fueled by methods of increasing resolution and/or throughput that strive to eliminate systematic biases and increase precision. In this review, we attempt to highlight the most recent advances in technology that promise to provide novel insights on how chromosomes fold and function.

中文翻译：

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研究空间基因组组织的技术：超越3C。

染色质在三维核空间中的组织方式现已被认为是对主要细胞过程（例如转录，复制和细胞分裂）至关重要的因素。研究人员已经配备了新的分子和成像技术，以研究基因组的这种结构与功能的联系。十多年前，由于引入了“染色体构象捕获”技术，该研究带头了。然而，该技术并非没有缺点，并且正在开发新颖的变体和正交方法来克服这些缺点。结果，不断提高分辨率和/或产量的方法不断为核组织领域提供动力，这些方法致力于消除系统性偏差并提高精度。在这篇评论中