Abstract The cationic copolymer poly(L-lysine)-graft-dextran (PLL-g-Dex) has nucleic acid chaperone-like activity. The copolymer facilitates both DNA hybridization and strand exchange reactions. For these reasons, DNA-based enzyme (DNAzyme) activity is enhanced in the presence of copolymer. In this study, we evaluated activities of DNAzymes with substrate-binding arms (S-arms) of various lengths. The copolymer promoted DNAzyme reactivity and turnover efficacy, and, depending on S-arm length, maximally accelerated the reaction rate by 250-fold compared to the rate in the absence of copolymer. The copolymer permitted up to six nucleotides truncation of the S-arms having initial length of 10 and 11 nucleotides without loss of catalytic efficiency, enable tuning of the optimal temperature ranging from 30 to 55 °C. The approach might be useful for the development of DNAzyme systems targeting short or highly structured RNAs as well for improvement of DNAzyme-based nanomachines and biosensors.

中文翻译：

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阳离子共聚物伴侣短臂 10-23 DNAzymes

摘要 阳离子共聚物聚（L-赖氨酸）-接枝葡聚糖（PLL-g-Dex）具有核酸分子伴侣样活性。该共聚物促进 DNA 杂交和链交换反应。由于这些原因，基于 DNA 的酶 (DNAzyme) 活性在共聚物的存在下增强。在这项研究中，我们评估了具有不同长度的底物结合臂（S 臂）的 DNAzyme 的活性。该共聚物促进了 DNAzyme 的反应性和转换效率，并且根据 S 臂长度，与没有共聚物时的反应速率相比，反应速率最大加快了 250 倍。该共聚物允许将初始长度为 10 和 11 个核苷酸的 S 臂截断多达 6 个核苷酸，而不会损失催化效率，从而能够在 30 至 55 °C 的范围内调节最佳温度。