This paper aims to demonstrate the efficacy of the immobilisation of the chemotherapy drug doxorubicin on nanodiamond platforms as a potential cancer therapy. This effective drug is experimentally fed into a human breast adenocarcinoma cell lines. Drug loading activity and cell viability are detected by spectrometer, microscopy, and MTT assay in this study at Biomedical Physics Research Unit, Department of Physics, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand between 1 Oct 2018 and 10 Jun 2019. Experimental results show that in the basic environment (pH = 8.0), the nanodiamond carboxylic group cooperated with the doxorubicin amino group to form a stable and non-covalent bond on nanodiamond surfaces served as a simple physical adsorption. In an acidic environment suitable to targeting the cancer cells, the nanodiamond carboxylic group ionised so that doxorubicin is effectively released. Doxorubicin therefore affirmatively absorbed into the cytoplasm and later into the nucleus. The significant finding of the study is that IC-50 equivalent to 0.40 mg/mL and viable nanodiamond-doxorubicin is a good candidate material for drug delivery.

中文翻译：

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纳米金刚石-阿霉素复合物对人乳腺癌细胞系的功效。

本文旨在证明将化学药物阿霉素固定在纳米金刚石平台上作为一种潜在的癌症治疗方法的有效性。通过实验将这种有效药物喂入人乳腺癌细胞系中。在这项研究中，通过分光光度计，显微镜和MTT分析检测了药物负载活性和细胞活力，该研究于2018年10月1日至10日在泰国曼谷拉德邦邦国王科技大学理学院物理系生物医学物理研究室10520之间进行2019年6月。实验结果表明，在碱性环境（pH = 8.0）下，纳米金刚石羧基与阿霉素氨基相互作用在纳米金刚石表面上形成稳定且非共价的键，可作为简单的物理吸附。在适合于靶向癌细胞的酸性环境中，纳米金刚石羧基离子化，从而有效释放了阿霉素。因此，阿霉素肯定地吸收到细胞质中，然后吸收到细胞核中。该研究的重要发现是，IC-50相当于0.40 mg / mL，并且是可行的纳米金刚石-阿霉素，是药物输送的良好候选材料。