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A Programming 20-30nm Rectangular DNA Origami for Loading Doxorubicin to Penetrate Ovarian Cancer Cells.
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2019-10-03 , DOI: 10.1109/tnb.2019.2943923 Xin Li , Xun Wang , Hong Li , Xiaolong Shi , Pan Zheng
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2019-10-03 , DOI: 10.1109/tnb.2019.2943923 Xin Li , Xun Wang , Hong Li , Xiaolong Shi , Pan Zheng
In DNA nanotechnology, the aim in folding DNA origami is to find a good piece of rectangular DNA origami with desired sizes, which could be larger or smaller for different application purposes. In recent three years, the technique of folding smaller ones is paid heavily attentions. In this work, we design a programming rectangular DNA origami in size 20*30nm with M13p18, which is smallest and cheapest to the best acknowledge of the authors. Since it is not hard to prepare with 30 staple strands and short annealing time, the cost of folding our designed rectangular DNA origami is less than 100 dollars. Although the large origami give more space, the smaller ones are cheaper and has the potential applications in penetrating cancer cells. It is obtained by cell penetrating experiments that our designed rectangular DNA origami can penetrate ovarian cancer cells efficiently even loading doxorubicin, but the thermodynamic stability needs further improved. Our designed programming 20 30nm triangular DNA origami shows potential applications in precision control of nanoscale particles and anti-tumor drug delivery in vivo.
中文翻译:
编程的20-30nm矩形DNA折纸,用于加载阿霉素以穿透卵巢癌细胞。
在DNA纳米技术中,折叠DNA折纸的目的是找到一块具有所需尺寸的矩形DNA折纸,为了不同的应用目的,该尺寸可以更大或更小。近三年来,折叠较小的技术备受关注。在这项工作中,我们用M13p18设计了大小为20 * 30nm的编程矩形DNA折纸,这最小,最便宜,这一点得到了作者的充分肯定。由于不难用30条短链准备并且退火时间短,因此折叠我们设计的矩形DNA折纸的成本不到100美元。尽管大的折纸提供了更多的空间,但较小的折纸却更便宜,并且在渗透癌细胞方面具有潜在的应用。通过细胞渗透实验,我们设计的矩形DNA折纸即使负载阿霉素也能有效穿透卵巢癌细胞,但热力学稳定性有待进一步提高。我们设计的程序设计20 30nm三角形DNA折纸展示了在纳米级颗粒的精确控制和体内抗肿瘤药物递送方面的潜在应用。
更新日期:2019-11-01
中文翻译:
编程的20-30nm矩形DNA折纸,用于加载阿霉素以穿透卵巢癌细胞。
在DNA纳米技术中,折叠DNA折纸的目的是找到一块具有所需尺寸的矩形DNA折纸,为了不同的应用目的,该尺寸可以更大或更小。近三年来,折叠较小的技术备受关注。在这项工作中,我们用M13p18设计了大小为20 * 30nm的编程矩形DNA折纸,这最小,最便宜,这一点得到了作者的充分肯定。由于不难用30条短链准备并且退火时间短,因此折叠我们设计的矩形DNA折纸的成本不到100美元。尽管大的折纸提供了更多的空间,但较小的折纸却更便宜,并且在渗透癌细胞方面具有潜在的应用。通过细胞渗透实验,我们设计的矩形DNA折纸即使负载阿霉素也能有效穿透卵巢癌细胞,但热力学稳定性有待进一步提高。我们设计的程序设计20 30nm三角形DNA折纸展示了在纳米级颗粒的精确控制和体内抗肿瘤药物递送方面的潜在应用。