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Economic production and biocontrol efficiency of lipopeptide biosurfactants from Bacillus mojavenis A21.
Biodegradation ( IF 3.6 ) Pub Date : 2018-12-06 , DOI: 10.1007/s10532-018-9864-7
Hanen Ben Ayed 1 , Manel Cheffi Azabou 2 , Noomen Hmidet 1 , Mohamed Ali Triki 2 , Moncef Nasri 1
Affiliation  

In the wake of an increased attention on the eco-friendly biopesticidal products and the rising market requirements for organic agents, lipopeptides compounds have been described as biological control agents which improve the overall health growth and development of plants. Nevertheless, their high production cost constitue the major flaw in their wide use to control plant diseases. The present article aims to formulate an economic media for lipopeptides production by Bacillus mojavensis A21 for application as natural fungicides for plant disease treatment. We herein demonstrated the suitability of the potato waste, as low cost substrate, for lipopeptides production. Moreover, sea water was found to be a good mineral salts sources. In the second part of this study, we investigate the inhibitory activity of A21 lipopeptides against the phtopathogenic Fusarium sp. The in vitro test showed a minimal inhibitory concentration of about 0.3 mg/ml. The microscopic examination, of the treated Fusarium revealed an excessive lysis of the mycelia ultrastructure with destructed spores. The in vivo antagonist activity was confirmed towards the infected potato tubers. A21 lipopeptides are effective in decreasing by about 78.26% and 60.68% when applied as preventive and curative treatments, respectively, as compared to the untreated tubers.

中文翻译:

Mojavenis A21脂肽生物表面活性剂的经济生产和生物防治效率。

随着人们越来越关注生态友好的生物农药产品以及对有机试剂的市场需求的增加,脂肽化合物已被描述为可改善植物整体健康生长和发育的生物防治剂。然而,它们的高生产成本构成了其广泛用于控制植物病害的主要缺陷。本文旨在为莫卡文芽孢杆菌生产脂肽的经济媒介奠定基础A21用作植物病害治疗的天然杀菌剂。我们在本文中证明了马铃薯废料作为低成本底物用于脂肽生产的适用性。此外,发现海水是良好的矿物盐来源。在本研究的第二部分中,我们研究了A21脂肽对光致病镰刀菌sp。的抑制活性。体外试验显示最小抑菌浓度约为0.3 mg / ml。经显微镜处理的镰刀菌病揭示了孢子破坏的菌丝体超微结构过度溶解。证实了对被感染马铃薯块茎的体内拮抗剂活性。与未经处理的块茎相比,A21脂肽作为预防和治疗剂分别有效减少约78.26%和60.68%。
更新日期:2018-12-06
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