Pseudomonas strains isolated from oil contaminated soils were screened for biosurfactant production. Three out of eleven Pseudomonas isolates were selected for their high emulsifying activity (E24 value on n-hexadecane ~ 78%). These isolates (E39, E311 and E313) were identified as members of the P. putida group using phenotypical methods and a molecular approach. To identify the chemical nature of produced biosurfactants, thin layer chromatography and MALDI-ToF mass spectrometry analysis were carried out and revealed lipopeptides belonging to the syringafactin family. The activity of the produced biosurfactants was stable over a pH range of 6–12, at high salinity (10%) and after heating at 80 °C. Tests in contaminated sand micro-bioreactors showed that the three strains were able to degrade diesel. These results suggest the potential of these syringafactin producing strains for application in hydrocarbon bioremediation.

中文翻译：

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丁香假单胞菌假单胞菌属spp的生物乳化和生物降解活性。从受油污染的土壤中分离的菌株。

从油污染的土壤中分离出的假单胞菌菌株用于生物表面活性剂的生产。从11个假单胞菌分离物中选择了3个具有高乳化活性（正十六烷的E24值为〜78％）。这些分离株（E39，E311和E313）被鉴定为恶臭假单胞菌的成员。组采用表型方法和分子方法。为了鉴定产生的生物表面活性剂的化学性质，进行了薄层色谱和MALDI-ToF质谱分析，并揭示了属于丁香脂素家族的脂肽。所生产的生物表面活性剂的活性在6-12的pH范围内，高盐度（10％）以及在80°C加热后稳定。在受污染的沙子微生物反应器中进行的测试表明，这三种菌株能够降解柴油。这些结果表明这些产生丁香脂素的菌株在烃生物修复中的应用潜力。