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Genomics of Early Cardiac Dysfunction and Mortality in Obese Drosophila melanogaster.
Physiological and Biochemical Zoology ( IF 1.6 ) Pub Date : 2019 , DOI: 10.1086/706099
James N. Kezos , Mark A. Phillips , Misty D. Thomas , Akamu J. Ewunkem , Grant A. Rutledge , Thomas T. Barter , Marta A. Santos , Brandon D. Wong , Kenneth R. Arnold , Laura A. Humphrey , Albert Yan , Chloe Nouzille , Isaias Sanchez , Larry G. Cabral , Timothy J. Bradley , Laurence D. Mueller , Joseph L. Graves , Michael R. Rose

In experimental evolution, we impose functional demands on laboratory populations of model organisms using selection. After enough generations of such selection, the resulting populations constitute excellent material for physiological research. An intense selection regime for increased starvation resistance was imposed on 10 large outbred Drosophila populations. We observed the selection responses of starvation and desiccation resistance, metabolic reserves, and heart robustness via electrical pacing. Furthermore, we sequenced the pooled genomes of these populations. As expected, significant increases in starvation resistance and lipid content were found in our 10 intensely selected SCO populations. The selection regime also improved desiccation resistance, water content, and glycogen content among these populations. Additionally, the average rate of cardiac arrests in our 10 obese SCO populations was double the rate of the 10 ancestral CO populations. Age-specific mortality rates were increased at early adult ages by selection. Genomic analysis revealed a large number of single nucleotide polymorphisms across the genome that changed in frequency as a result of selection. These genomic results were similar to those obtained in our laboratory from less direct selection procedures. The combination of extensive genomic and phenotypic differentiation between these 10 populations and their ancestors makes them a powerful system for the analysis of the physiological underpinnings of starvation resistance.

中文翻译:

肥胖果蝇早期心脏功能障碍和死亡率的基因组学。

在实验演变中,我们对使用选择的模型生物的实验室种群提出了功能要求。经过足够多代的选择,所得的种群构成了用于生理学研究的极佳材料。对10个大型近交果蝇种群施加了强烈的选择机制,以增加抗饥饿性。通过电起搏,我们观察到饥饿和耐干燥性,代谢储备和心脏健壮性的选择反应。此外,我们对这些种群的合并基因组进行了测序。不出所料,在我们的10个强烈选择的SCO人群中,抗饥饿性和脂质含量显着增加。选择方案还改善了这些种群的抗干燥性,水分含量和糖原含量。另外,我们10个肥胖SCO人群的平均心脏骤停率是10个祖先CO人群的两倍。通过选择,成年早期的特定年龄死亡率增加。基因组分析揭示了整个基因组中大量的单核苷酸多态性,这些多态性由于选择而发生频率变化。这些基因组结果与在我们实验室中通过较少直接选择程序获得的结果相似。这10个种群及其祖先之间广泛的基因组和表型分化相结合,使它们成为分析饥饿抗性生理基础的强大系统。通过选择,成年早期的特定年龄死亡率增加。基因组分析揭示了整个基因组中大量的单核苷酸多态性,这些多态性由于选择而发生频率变化。这些基因组结果与在我们实验室中通过较少直接选择程序获得的结果相似。这10个种群及其祖先之间广泛的基因组和表型分化相结合,使它们成为分析饥饿抗性生理基础的强大系统。通过选择,成年早期的特定年龄死亡率增加。基因组分析揭示了整个基因组中大量的单核苷酸多态性,这些多态性由于选择而发生频率变化。这些基因组结果与在我们实验室中通过较少直接选择程序获得的结果相似。这10个种群及其祖先之间广泛的基因组和表型分化相结合,使它们成为分析饥饿抗性生理基础的强大系统。
更新日期:2019-11-01
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