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Genetic diversity and population structure of Culex modestus across Europe: does recent appearance in the United Kingdom reveal a tendency for geographical spread?
Medical and Veterinary Entomology ( IF 1.9 ) Pub Date : 2019-10-11 , DOI: 10.1111/mve.12412
L M Hernández-Triana 1 , V A Brugman 2, 3 , P Pramual 4 , E Barrero 1 , N I Nikolova 5 , I Ruiz-Arrondo 6 , A Kaiser 7 , A Krüger 8 , S Lumley 9 , H C Osório 10 , A Ignjatović-Ćupina 11 , D Petrić 12 , M Laure Setier-Rio 12 , R Bødker 13 , N Johnson 1, 14
Affiliation  

In mainland Europe, the mosquito species Culex modestus Ficalbi (1890) is a bridge vector for West Nile virus (WNV) from its natural bird-mosquito cycle to mammals. The present study assessed the genetic diversity of Cx. modestus, as well as related Culex species, using the mitochondrial COI DNA barcoding region and compared this with the population structure across Europe. A haplotype network was mapped to determine genealogical relationships among specimens. The intraspecific genetic diversity within individual Culex species was below 2%, whereas the interspecific genetic divergence varied from 2.99% to 13.74%. In total, 76 haplotypes were identified among 198 sequences. A median-joining network determined from 198 COI sequences identified two major lineages that were separated by at least four mutation steps. A high level of intraspecific genetic diversity was not detected in Cx. modestus in samples submitted from different European populations, which indicates that morphologically identified specimens represent a single species and not a species complex. Therefore, it is deduced that different populations of Cx. modestus will show a similar potential to transmit WNV, lending support to concerns that the population present in southeast England represents a risk of transmission to humans.

中文翻译:

欧洲库蚊的遗传多样性和种群结构:最近在英国的出现是否显示了地理扩散的趋势?

在欧洲大陆,蚊子库克斯模蝇Ficalbi(1890)是西尼罗河病毒(WNV)从其自然鸟类-蚊子传播到哺乳动物的桥梁载体。本研究评估了Cx的遗传多样性。使用线粒体COI DNA条形码区域的模式鸟以及相关库蚊种,并将其与欧洲的人口结构进行比较。单倍型网络被映射以确定标本之间的族谱关系。单个库蚊种内的种内遗传多样性低于2%,而种间遗传多样性从2.99%到13.74%不等。总共在198个序列中鉴定出76个单倍型。由198个COI序列确定的中位连接网络确定了两个主要谱系,这些谱系由至少四个突变步骤隔开。在Cx中未检测到高水平的种内遗传多样性。从不同欧洲种群提交的样品中发现了模式昆虫,这表明从形态学上鉴定的标本代表单个物种,而不是物种复合体。因此,推断出不同的Cx种群。方式将显示出传播西尼韦尔病毒的类似潜力,这为人们担心英格兰东南部的人口存在向人类传播的风险提供了支持。
更新日期:2019-10-11
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