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Microbiota characterization of sheep milk and its association with somatic cell count using 16s rRNA gene sequencing
Journal of Animal Breeding and Genetics ( IF 2.6 ) Pub Date : 2019-10-10 , DOI: 10.1111/jbg.12446
Cristina Esteban-Blanco 1 , Beatriz Gutiérrez-Gil 1 , Fernando Puente-Sánchez 2 , Héctor Marina 1 , Javier Tamames 2 , Alberto Acedo 3 , Juan José Arranz 1
Affiliation  

This work aimed to use 16S ribosomal RNA sequencing with the Illumina MiSeq platform to describe the milk microbiota from 50 healthy Assaf ewes. The global observed microbial community for clinically healthy milk samples analysed was complex and showed a vast diversity. The core microbiota of the sheep milk includes five genera: Staphylococcus, Lactobacillus, Corynebacterium, Streptococcus and Escherichia/Shigella. Although there are some differences, some of these genera are common with the microbiota core pattern of milk from other species, especially with dairy cows. The microbial composition of the studied samples, based on the definition of amplicon sequence variants, was analysed through a correlation network. A preliminary analysis by grouping the milk samples based on their somatic cell count (SCC), which is considered an indicator of subclinical mastitis (SM), showed certain differences for the core of the samples identified as SM. The differences in the microbiota diversity pattern among samples might also suggest that subclinical mastitis would be associated with the significant increase in some genera that are inhabitants of the mammary gland and a remarkable concomitant reduction in the microbial diversity. Additionally, we have also presented here a preliminary analysis to assess the impact of the sheep milk microbiome on SCC, as an indicator of subclinical mastitis. The results here reported provide a first characterization of the sheep milk microbiota and settle the basis for future studies in this field.

中文翻译:

羊奶微生物群特征及其与体细胞计数的 16s rRNA 基因测序的关联

这项工作旨在使用 16S 核糖体 RNA 测序和 Illumina MiSeq 平台来描述来自 50 只健康阿萨夫母羊的牛奶微生物群。所分析的临床健康牛奶样品的全球观察到的微生物群落很复杂,并且表现出巨大的多样性。羊奶的核心微生物群包括五个属:葡萄球菌属、乳酸杆菌属、棒状杆菌属、链球菌属和埃希氏菌属/志贺氏菌属。尽管存在一些差异,但其中一些属与来自其他物种的牛奶的微生物群核心模式相同,尤其是奶牛。基于扩增子序列变体的定义,研究样品的微生物组成通过相关网络进行分析。根据体细胞计数 (SCC) 对牛奶样品进行分组的初步分析,这被认为是亚临床乳腺炎 (SM) 的一个指标,对于鉴定为 SM 的样品的核心显示出某些差异。样本间微生物群多样性模式的差异也可能表明,亚临床乳腺炎可能与乳腺中某些属的显着增加以及微生物多样性的显着减少有关。此外,我们还在此处进行了初步分析,以评估羊奶微生物群对 SCC 的影响,作为亚临床乳腺炎的指标。这里报告的结果提供了羊奶微生物群的第一个特征,并为该领域的未来研究奠定了基础。样本间微生物群多样性模式的差异也可能表明,亚临床乳腺炎可能与乳腺中某些属的显着增加以及微生物多样性的显着减少有关。此外,我们还在此处进行了初步分析,以评估羊奶微生物群对 SCC 的影响,作为亚临床乳腺炎的指标。这里报告的结果提供了羊奶微生物群的第一个特征,并为该领域的未来研究奠定了基础。样本间微生物群多样性模式的差异也可能表明,亚临床乳腺炎可能与乳腺中某些属的显着增加以及微生物多样性的显着减少有关。此外,我们还在此处进行了初步分析,以评估羊奶微生物群对 SCC 的影响,作为亚临床乳腺炎的指标。这里报告的结果提供了羊奶微生物群的第一个特征,并为该领域的未来研究奠定了基础。我们还在此介绍了一项初步分析,以评估羊奶微生物群对 SCC 的影响,作为亚临床乳腺炎的指标。这里报告的结果提供了羊奶微生物群的第一个特征,并为该领域的未来研究奠定了基础。我们还在此介绍了一项初步分析,以评估羊奶微生物群对 SCC 的影响,作为亚临床乳腺炎的指标。这里报告的结果提供了羊奶微生物群的第一个特征,并为该领域的未来研究奠定了基础。
更新日期:2019-10-10
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