Cell division is accompanied by dramatic changes in shape that ultimately lead to the physical separation of one cell into two. In 2D microenvironments, cells round up and remain adhered onto the substrate by thin retraction fibers during division. In contrast, in 3D environments, cells divide exhibiting long protrusions that guide the orientation of the division axis. However, the mechanism of cell division in three dimensions still remains poorly understood. Here we report the spontaneous formation of transient quasiperiodic membrane pearling on extended mitotic protrusions during 3D cell division. Protrusion membrane pearling may be initiated by the non-uniform distribution of focal adhesions and consequent stationary instability of the protrusive membrane. Overall, membrane pearling emergence may provide insights into a novel modality of 3D cell division with potential physiological relevance.

中文翻译：

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在3D细胞分裂过程中会出现突起的膜珠光。

细胞分裂伴随着形状的急剧变化，最终导致将一个细胞物理分离为两个。在2D微环境中，细胞在分裂过程中会聚集并且通过细小的回缩纤维保持粘附在基底上。相反，在3D环境中，细胞分裂时会呈现出长的突起，这些突起会引导划分轴的方向。但是，在三个维度上细胞分裂的机制仍然知之甚少。在这里，我们报告在3D细胞分裂过程中在扩展的有丝分裂突起上瞬时形成的准周期性膜珠光的自发形成。突出膜的珠光起因于粘着斑的不均匀分布以及由此引起的突出膜的固定不稳定性。总体，