Pig-induced pluripotent stem cells (piPSCs) have great potential application in regenerative medicine. The miR-302s cluster alone has been shown to reprogram mouse and human somatic cells into induced pluripotent stem cells (iPSCs) without exogenous transcription factors. However, miR-302s alone have not been reported to reprogram cells in large livestock. In this study, we induced pig somatic cells into partially reprogrammed piPSCs using overexpression of the miR-302s cluster (miR-302s-piPSC) and investigated the early reprogramming events during the miRNA induction process. The results showed that miR-302s-piPSCs exhibited some characteristics of pluripotent stem cells including expression of pluripotency markers-particularly, efficient activation of endogenous OCT4-and differentiation to the three germ layers in vitro. During the early reprogramming process, somatic cells first underwent epithelial-mesenchymal transition and then mesenchymal-epithelial transition to eventually form miR-302s-piPSCs. These data show, for the first time, that single factor miR-302s successfully induced pig somatic cells into miR-302s-piPSCs. This study provides a new tool and research direction for the induction of pluripotent stem cells in a large livestock.

中文翻译：

---

在广西巴马小型猪成纤维细胞中使用MicroRNA簇miR-302s部分重编程诱导多能干细胞。

猪诱导的多能干细胞（piPSC）在再生医学中具有巨大的潜在应用。已显示，仅miR-302s簇可将小鼠和人类体细胞重编程为诱导型多能干细胞（iPSC），而无需外源转录因子。但是，尚未报道仅miR-302能够重编程大型牲畜中的细胞。在这项研究中，我们使用miR-302s簇（miR-302s-piPSC）的过表达将猪体细胞诱导为部分重编程的piPSC，并研究了miRNA诱导过程中的早期重编程事件。结果表明，miR-302s-piPSCs具有多能干细胞的一些特征，包括多能性标志物的表达-特别是内源性OCT4的有效激活和体外向三个胚层的分化。在早期的重新编程过程中，体细胞首先经历上皮-间质转化，然后经历间质-上皮转化，最终形成miR-302s-piPSC。这些数据首次显示，单因子miR-302s成功地将猪体细胞诱导为miR-302s-piPSC。这项研究为诱导大型牲畜的多能干细胞提供了新的工具和研究方向。