Easy and visually appealing demonstrations are precious tools for introducing students to the study of enzymes. However, they most often involve purified enzymes and dedicated techniques. Here, we propose a set of demonstrations, which require only fresh horseradish root and consumer chemicals, to help support biochemistry and enzymology courses from high school to the undergraduate level. Horseradish root is a naturally rich source of horseradish peroxidase, an enzyme with many relevant practical applications. Slices of horseradish root are used to demonstrate the characteristic reaction of horseradish peroxidase with hydrogen peroxide, the selective inhibition of this enzyme by dilute hydrochloric acid, its chromogenic reaction with N,N′‐diethyl‐p‐phenylenediamine, and its ability to trigger the chemiluminescent reaction of luminol. © 2019 International Union of Biochemistry and Molecular Biology, 48(1):38–43, 2020.

中文翻译：

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辣根微笑：以视觉上吸引人的方式展示过氧化物酶的特征反应

简单且具有视觉吸引力的演示是向学生介绍酶研究的宝贵工具。然而，它们通常涉及纯化酶和专用技术。在这里，我们提出了一组仅需要新鲜辣根和消费化学品的演示，以帮助支持从高中到本科阶段的生物化学和酶学课程。辣根是辣根过氧化物酶的天然丰富来源，辣根过氧化物酶是一种具有许多相关实际应用的酶。辣根根切片用于证明辣根过氧化物酶与过氧化氢的特征反应，稀盐酸对该酶的选择性抑制，其与 N,N'-二乙基-对苯二胺的显色反应，以及其触发过氧化氢的能力鲁米诺的化学发光反应。