Noninvasive imaging techniques provide insight into physiology that is complementary to tissue morphology obtained by invasive histology. Optical imaging techniques, such as laser speckle contrast analysis, are used in vivo to longitudinally evaluate vascularization. Despite their high spatial resolution, these techniques have a limited imaging depth. In this study, we demonstrate how a dual LED-based photoacoustic (PA) and ultrasound system can delineate changes in perfusion at depth within scaffolds containing basic fibroblast growth factor. Perfusion changes detected by PA corroborated with vessel density. PA imaging could be a noninvasive and sensitive method for evaluating vascularization at depth in larger constructs.

中文翻译：

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基于LED的光声成像技术可监测血纤蛋白支架中的血管生成。

非侵入性成像技术提供了对生理学的见识，该生理学与通过侵入性组织学获得的组织形态互补。在体内使用光学成像技术（例如激光散斑对比度分析）来纵向评估血管形成。尽管它们的空间分辨率很高，但是这些技术的成像深度有限。在这项研究中，我们演示了基于双LED的光声（PA）和超声系统如何描绘包含碱性成纤维细胞生长因子的支架内深度处的灌注变化。PA检测到的灌注变化与血管密度有关。PA成像可能是一种无创且敏感的方法，用于评估较大结构中的深度血管形成。