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Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior.
Cellular and Molecular Bioengineering ( IF 2.8 ) Pub Date : 2018-06-01 , DOI: 10.1007/s12195-018-0533-z
Christopher S Lewis 1 , Nesreen Z Alsmadi 2 , Trevor A Snyder 3 , David W Schmidtke 2
Affiliation  

Introduction

Neutrophils display an array of behaviors ranging from rolling and migration to phagocytosis and granule secretion. Several of these behaviors are modulated by the local shear conditions. In the normal circulation, neutrophils experience shear rates from approximately 10–2000 s−1. However, neutrophils are also exposed to pathological shear levels in natural conditions such as severe stenosis and arteriosclerosis, as well as in blood-contacting devices such as ventricular assist devices and hemodialysis machines. The effects of transiently (< 1 s) exposing neutrophils to abnormally high shear rates (> 3000 s−1) are not well understood.

Methods

We developed a set of microfluidic devices capable of exposing neutrophils to high shear rates for short durations (100–400 ms). Suspensions of isolated neutrophils were perfused through the devices and their rolling velocities on P-selectin were analyzed before and after shear exposure.

Results

We observed a significant increase in neutrophil rolling velocities on P-selectin coated regions following transient high shear exposure. The magnitude of the rolling velocity increase was dependent upon the duration of high shear exposure and became statistically significant for exposure times of 310 ms or longer. When polystyrene beads coated with a glycosulfopeptide that mimics the binding region of P-selectin glycoprotein ligand-1 (PSGL-1) were perfused through the devices, no change between the pre-shear and post-shear rolling velocities was observed.

Conclusions

These results suggest that high shear levels alter normal neutrophil rolling behavior and are important for understanding neutrophil biology in high shear conditions, as well as for improving medical device performance.


中文翻译:

瞬时暴露于高剪切对中性粒细胞滚动行为的影响。

介绍

中性粒细胞表现出一系列行为,从滚动和迁移到吞噬和颗粒分泌。其中一些行为受到局部剪切条件的调节。在正常循环中,中性粒细胞经历大约 10-2000 s -1的剪切速率。然而,在严重狭窄和动脉硬化等自然条件下,以及在心室辅助装置和血液透析机等血液接触装置中,中性粒细胞也会受到病理性剪切水平的影响。暂时(< 1 s)将中性粒细胞暴露于异常高剪切速率(> 3000 s -1)的影响尚不清楚。

方法

我们开发了一套微流体装置,能够在短时间内(100-400 毫秒)将中性粒细胞暴露在高剪切率下。分离的中性粒细胞悬浮液通过装置灌注,并在剪切暴露前后分析它们在 P-选择素上的滚动速度。

结果

我们观察到瞬态高剪切暴露后 P-选择素涂层区域的中性粒细胞滚动速度显着增加。滚动速度增加的幅度取决于高剪切暴露的持续时间,并且对于 310 ms 或更长的暴露时间变得具有统计学意义。当用模拟 P-选择素糖蛋白配体-1 (PSGL-1) 的结合区域的糖硫肽包被的聚苯乙烯珠子灌注通过装置时,未观察到剪切前和剪切后滚动速度之间的变化。

结论

这些结果表明,高剪切水平会改变正常的中性粒细胞滚动行为,对于了解高剪切条件下的中性粒细胞生物学以及改善医疗器械性能非常重要。
更新日期:2018-06-01
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