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Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior.
Cellular and Molecular Bioengineering ( IF 2.8 ) Pub Date : 2018-06-01 , DOI: 10.1007/s12195-018-0533-z Christopher S Lewis 1 , Nesreen Z Alsmadi 2 , Trevor A Snyder 3 , David W Schmidtke 2
中文翻译:
瞬时暴露于高剪切对中性粒细胞滚动行为的影响。
更新日期:2018-06-01
Cellular and Molecular Bioengineering ( IF 2.8 ) Pub Date : 2018-06-01 , DOI: 10.1007/s12195-018-0533-z Christopher S Lewis 1 , Nesreen Z Alsmadi 2 , Trevor A Snyder 3 , David W Schmidtke 2
Affiliation
Introduction
Neutrophils display an array of behaviors ranging from rolling and migration to phagocytosis and granule secretion. Several of these behaviors are modulated by the local shear conditions. In the normal circulation, neutrophils experience shear rates from approximately 10–2000 s−1. However, neutrophils are also exposed to pathological shear levels in natural conditions such as severe stenosis and arteriosclerosis, as well as in blood-contacting devices such as ventricular assist devices and hemodialysis machines. The effects of transiently (< 1 s) exposing neutrophils to abnormally high shear rates (> 3000 s−1) are not well understood.Methods
We developed a set of microfluidic devices capable of exposing neutrophils to high shear rates for short durations (100–400 ms). Suspensions of isolated neutrophils were perfused through the devices and their rolling velocities on P-selectin were analyzed before and after shear exposure.Results
We observed a significant increase in neutrophil rolling velocities on P-selectin coated regions following transient high shear exposure. The magnitude of the rolling velocity increase was dependent upon the duration of high shear exposure and became statistically significant for exposure times of 310 ms or longer. When polystyrene beads coated with a glycosulfopeptide that mimics the binding region of P-selectin glycoprotein ligand-1 (PSGL-1) were perfused through the devices, no change between the pre-shear and post-shear rolling velocities was observed.Conclusions
These results suggest that high shear levels alter normal neutrophil rolling behavior and are important for understanding neutrophil biology in high shear conditions, as well as for improving medical device performance.中文翻译:
瞬时暴露于高剪切对中性粒细胞滚动行为的影响。