BACKGROUND Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood. OBJECTIVE We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue. METHODS MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 μm pore filters coated with the same proteins was analysed. RESULTS The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSC>BMMSC>TBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSC>TBMSC≥UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC. CONCLUSIONS MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.

中文翻译：

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来自不同组织的间充质干细胞的比较粘附和迁移特性。

背景技术间充质干细胞(MSC)用于治疗，通常通过注射到血液中。目的 我们旨在比较脐带 (UCMSC)、骨髓 (BMMSC) 或小梁骨 (TBMSC) 的 MSC 的粘附和迁移特性，这可能会影响向受伤组织的输送。方法 MSC 通过涂有基质蛋白、胶原蛋白或纤连蛋白或白蛋白的玻璃毛细管灌注。用显微镜对贴壁细胞进行计数，并随时间分析它们的扩散。分析了通过涂有相同蛋白质的 8 μm 孔过滤器的 MSC 迁移。结果黏附在胶原蛋白上的MSC数量大于纤连蛋白，随着壁面剪切速率从17~70 s-1增加而减少，其大小顺序为UCMSC>BMMSC>TBMSC。相反，扩散对纤连蛋白更有效，并且顺序为 BMMSC> TBMSC≥UCMSC。通过用任一基质蛋白涂覆过滤器的下表面来促进迁移，UCMSC的迁移比BMMSC更有效。结论 MSC 在其从流动中捕获的效率和随后的扩散或在基质蛋白上迁移的能力方面显示出依赖于起源的变化。UCMSC 显示出最有效的流动捕获，其次是较少的扩散，但更快速的迁移。这些反应可能与更有效地从循环传递到受损组织有关。UCMSC 显示出最有效的流动捕获，其次是较少的扩散，但更快速的迁移。这些反应可能与更有效地从循环传递到受损组织有关。UCMSC 显示出最有效的流动捕获，其次是较少的扩散，但更快速的迁移。这些反应可能与更有效地从循环传递到受损组织有关。