The introduction to high-speed countercurrent chromatography of a spiral tubing support rotor has enabled the application of more polar volatile solvent systems for natural products separation, especially water soluble compounds and their metabolites. Here is reported the use of spiral countercurrent chromatography with the spiral tubing support rotor to fractionate extracts of the African medicinal plant Sutherlandia frutescens (L.) R.Br. A two-phase solvent system of ethyl acetate, methanol, water, and n-butanol, with the lower aqueous phase mobile, separated sutherlandioside B efficiently from other compounds. The purity of sutherlandioside B was determined by high-performance liquid chromatography and mass spectrometry analysis. Sufficient quantities of sutherlandioside B can be purified by this method for use in cell culture assays and the development of a sensitive antibody by which to detect the consumption and metabolism in vivo of this novel botanical.

中文翻译：

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从 Sutherlandia frutescens(L.) R.Br. 中分离主要的环阿烷糖苷，Sutherlandioside B。通过螺旋逆流色谱

螺旋管支撑转子高速逆流色谱的引入使极性更大的挥发性溶剂系统能够应用于天然产物分离，尤其是水溶性化合物及其代谢物。这里报道了使用螺旋管支撑转子的螺旋逆流色谱分离非洲药用植物 Sutherlandia frutescens (L.) R.Br 的提取物。乙酸乙酯、甲醇、水和正丁醇的两相溶剂系统，具有较低的水相流动性，可有效地将 sutherlandioside B 与其他化合物分离。sutherlandioside B 的纯度通过高效液相色谱和质谱分析确定。