当前位置: X-MOL 学术Curr. Opin. Genet. Dev. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Reprogramming somatic cells towards pluripotency by cellular fusion.
Current Opinion in Genetics & Development ( IF 4 ) Pub Date : 2012-08-08 , DOI: 10.1016/j.gde.2012.07.005
Jorge Soza-Ried 1 , Amanda G Fisher
Affiliation  

Pluripotent cells arise within the inner cell mass (ICM) of mammals and have the potential to generate all cell types of the adult organism through a process of commitment and ordered differentiation. Despite many decades of investigation, the mechanisms that guide and stabilise cell fate choice as well as those that can be engineered to promote its reversal, remain only partially resolved. Reprogramming of somatic cells towards a pluripotent-like state can be achieved by several different experimental routes including nuclear transfer, the supply of a defined cocktail of transcription factors, or by fusing somatic cells with a pluripotent stem cell partner. These approaches have been used to demonstrate the remarkable intrinsic epigenetic plasticity of many terminally differentiated cell types, as well as to define the factors that are required for pluripotent conversion. In this review we summarise some recent advances using cell fusion-based studies to better understand the basis of pluripotency and the epigenetic mechanisms that promote cell type inter-conversion.

中文翻译:

通过细胞融合将体细胞重编程为多能性。

多能细胞出现在哺乳动物的内细胞群 (ICM) 中,并有可能通过定型和有序分化过程产生成年生物体的所有细胞类型。尽管进行了数十年的研究,但指导和稳定细胞命运选择的机制以及可以通过工程改造以促进其逆转的机制仍然只是部分得到解决。将体细胞重编程为多能样状态可以通过几种不同的实验途径来实现,包括核移植、提供特定的转录因子混合物或通过将体细胞与多能干细胞伴侣融合。这些方法已被用于证明许多终末分化细胞类型的显着内在表观遗传可塑性,以及定义多能转换所需的因素。在这篇综述中,我们总结了一些使用基于细胞融合的研究的最新进展,以更好地理解多能性的基础和促进细胞类型相互转换的表观遗传机制。
更新日期:2012-08-03
down
wechat
bug