Using a mouse neuroblastoma cell line, we have demonstrated that vaccination of tumor-free mice with a cell-based vaccine leads to productive immunity and resistance to tumor challenge, while vaccination of tumor-bearing mice does not. The T cell immunity induced by this vaccine, as measured by in vitro assays, is amplified by the depletion of Treg. Our goal is to understand this barrier to the development of protective cellular immunity. mRNA microarray analyses of CD8(+) T cells from naïve or tumor-bearing mice undergoing vaccination were carried out with or without administering anti-CD25 antibody. Gene-expression pathway analysis revealed the presence of CD8(+) T cells expressing stem cell-associated genes early after induction of productive anti-tumor immunity in tumor-free mice, prior to any phenotypic changes, but not in tumor-bearing mice. These data demonstrate that early after the induction of productive immune response, cells within the CD8(+) T cell compartment adopt a stem cell-related genetic phenotype that correlates with increased anti-tumor function.

中文翻译：

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用肿瘤疫苗治疗后干细胞相关基因在 CD8 区室中的早期表达。

使用小鼠神经母细胞瘤细胞系，我们已经证明用基于细胞的疫苗接种无肿瘤小鼠会导致产生免疫性和对肿瘤攻击的抵抗力，而接种荷瘤小鼠则不会。通过体外试验测量，这种疫苗诱导的 T 细胞免疫会因 Treg 的消耗而被放大。我们的目标是了解这种保护性细胞免疫发展的障碍。对来自接受疫苗接种的幼稚或荷瘤小鼠的 CD8(+) T 细胞进行 mRNA 微阵列分析，无论是否使用抗 CD25 抗体。基因表达通路分析显示，在任何表型变化之前，在无肿瘤小鼠中诱导生产性抗肿瘤免疫后，早期存在表达干细胞相关基因的 CD8(+) T 细胞，但在荷瘤小鼠中则不然。