Preparative high-speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of caffeic acid from Eupatorium adenophorum Spreng with a solvent system composed of ethyl acetate-methanol-water at a volume ratio of 10:1:10, v/v. Using a preparative unit of the HSCCC centrifuge, about a 938 mg amount of the crude extract was separated, yielding 63.2 mg of caffeic acid at purity of 96.0%. Then, the antimicrobial and antivirus drug caffeic acid (C9H8O4) was intercalated into layered double hydroxides for the first time by anion exchange under a nitrogen atmosphere. The product caffeic acid–LDH has been characterized by powder X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and Scanning electron micrographs (SEM), indicating that the drug has been successfully intercalated into LDH.

中文翻译：

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高速逆流色谱法从深紫草中分离咖啡酸并合成咖啡酸插层双氢氧化物

制备型高速逆流色谱 (HSCCC) 成功地用于从紫茎泽兰中分离纯化咖啡酸，溶剂体系为体积比为 10:1:10 (v/v) 的乙酸乙酯-甲醇-水。使用HSCCC离心机的制备单元，分离了约938mg量的粗提物，产生63.2mg纯度为96.0%的咖啡酸。然后，抗菌和抗病毒药物咖啡酸（C9H8O4）首次在氮气氛下通过阴离子交换嵌入层状双氢氧化物。产品咖啡酸-LDH 已通过粉末 X 射线衍射 (XRD)、傅里叶变换红外 (FT-IR) 和扫描电子显微照片 (SEM) 进行表征，表明该药物已成功嵌入 LDH。