The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.

中文翻译：

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定量树毛小球藻和嗜盐小球藻的生存力。

壶菌病是全球两栖动物数量下降的原因。霉菌病是由生活时间稳定和可传播的真菌病原体Batrachochytrium dendrobatidis（Bd）和B. salamandrivorans（Bsal）引起的。建立量化两个生命阶段的生长和存活的方法可以促进对这些病原体的病理生理学和疾病生态学的研究。我们测试了MTT分析（细胞活力的比色测试）的功效，发现它是定量Bd和Bsal活力的可靠方法固定的生活阶段。这种方法可以洞悉这些病原体的生长和繁殖，从而增进我们对壶菌的认识。