Acyl carrier proteins (ACPs) are important components in fatty‐acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.

中文翻译：

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Rv0100，结核分枝杆菌中拟议的酰基载体蛋白：表达、纯化和结晶。

酰基载体蛋白（ACP）是原核生物脂肪酸生物合成的重要组成部分。Rv0100预计是结核病病原体结核分枝杆菌中的重要ACP，因此有可能成为新型抗结核药物靶点。在此，报道了使用耻垢分枝杆菌作为宿主成功克隆和纯化 Rv0100 。获得的纯化蛋白质晶体的衍射分辨率为 1.9 Å。总的来说，这项工作为未来针对这一潜在新药物靶点的药物发现和开发奠定了基础。