Malignant Melanoma is known for being one of the most aggressive cancers with an incidence that increases every year. Cancer stem cells (CSCs) are involved in the resistance to therapeutic treatments, the metastatic process, and the patient's relapses. Thus, it is of vital importance for researchers to find the methodology that allows us to obtain enriched subpopulations that maintain their stem-like properties without differentiating over time. In the present manuscript, our objective was to compare the ability of conditioned medium obtained from human mesenchymal stem cells (MSCs) isolated by enzymatic and non-enzymatic methods for the enrichment and maintenance of melanoma CSCs. Our results showed for the first time that MSCs isolated by less aggressive methodology displayed higher CSCs enrichment and maintenance capacity. Because they do not undergo enzymatic and proteolytic stress, MSCs produce a greater amount of signalling molecules, helping to improve the phenotype characteristics of CSCs and keep them over time.

中文翻译：

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黑色素瘤癌干样细胞：培养，富集和维持的优化方法。

恶性黑色素瘤是最凶猛的癌症之一，其发病率每年都在增加。癌症干细胞（CSC）参与了对治疗方法的抵抗，转移过程以及患者的复发。因此，对于研究人员而言至关重要的是，找到一种方法，使我们能够获得丰富的亚群，这些亚群能够保持其茎状特性而不会随时间而分化，这一点至关重要。在本文中，我们的目标是比较从通过酶促和非酶促方法分离的人间充质干细胞（MSC）获得的条件培养基对黑色素瘤CSC富集和维持的能力。我们的结果首次显示，通过较少攻击性方法分离的MSC表现出更高的CSC富集和维持能力。