Cell-based therapy provides a promising approach for the treatment of sepsis and related disorders. Fate determination of transplanted cells is the most essential issue for cell therapist. Optical imaging is the reliable, time and cost-effective system for cell tracking. The present study was aimed to apply an optical imaging system for monitoring of GFP-labeled unrestricted somatic stem cells in the sepsis animal model. In vivo imaging showed the most accumulation of intravenously injected cells into the lungs and liver of septic mice. Thereafter, the imaging data were more approved by flow cytometry and immunohistochemistry staining. Cellular localization in septic lungs and liver that observed by optical imaging technique may offer beneficial evidence for designing of sepsis clinical trials.

中文翻译：

---

追踪在脓毒症小鼠模型中移植的GFP标记的无限制体细胞。

基于细胞的疗法为脓毒症和相关疾病的治疗提供了一种有希望的方法。决定移植细胞的命运是细胞治疗师最重要的问题。光学成像是用于细胞跟踪的可靠，省时且经济高效的系统。本研究旨在将光学成像系统应用于败血症动物模型中GFP标记的非限制性体细胞的监测。体内成像显示，静脉内注射的细胞向脓毒症小鼠的肺和肝中积累最多。此后，通过流式细胞术和免疫组织化学染色更认可了成像数据。通过光学成像技术观察到的化脓性肺和肝细胞定位可能为脓毒症临床试验的设计提供有益的证据。