Tumor energy metabolism and angiogenesis play significant roles in tumor genesis and development, while the effect of the tumor microenvironment (TME), which tumors rely on, is always ignored. In this research, we cocultured bladder cancer (BC) T24 cells with tumor‐associated human umbilical vein endothelial cells (HUVECs) under normoxic and hypoxic conditions and detected proliferation, migration, oxidative phosphorylation (OXPHOS) and glycolysis to reveal the energy metabolism characteristics and their effect on cell biological behaviors (CBBs) in the TME. Compared with single‐cultured cells, both cocultured T24 cells and HUVECs showed poor proliferation and migration in hypoxic environment, and OXPHOS was activated in cocultured T24 cells but weakened in cocultured HUVECs. However, in normoxic environment, cocultured T24 cells grew much faster while cocultured HUVECs grew slower compared with single‐cultured cells. Additionally, glycolysis played a crucial role in energy metabolism and was inhibited in cocultured T24 cells but activated in cocultured HUVECs. In normoxic TME, OXPHOS take main responsibility of energy metabolism. T24 cells exhibited increased proliferation and migration with HUVECs support. In hypoxic TME, glycolysis may be the primary energy supply pathway. T24 cells then exhibit suppressed proliferation and migration, while HUVECs tend to promote angiogenesis to adapt to the harsh TME.

中文翻译：

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肿瘤微环境对膀胱癌细胞和内皮细胞的细胞生物学行为的影响引起能量代谢的变化。

肿瘤能量代谢和血管生成在肿瘤的发生和发展中起着重要作用，而肿瘤所依赖的肿瘤微环境（TME）的作用始终被忽略。在这项研究中，我们在缺氧和缺氧条件下将膀胱癌（BC）T24细胞与肿瘤相关的人脐静脉内皮细胞（HUVEC）共培养，并检测了增殖，迁移，氧化磷酸化（OXPHOS）和糖酵解，以揭示能量代谢特征和它们对TME中细胞生物学行为（CBB）的影响。与单培养细胞相比，共培养的T24细胞和HUVEC在缺氧环境中均显示出较差的增殖和迁移，OXPHOS在共培养的T24细胞中被激活，但在共培养的HUVEC中被减弱。但是，在常氧环境下，与单培养细胞相比，共培养的T24细胞生长快得多，而共培养的HUVEC生长慢。另外，糖酵解在能量代谢中起关键作用，在共培养的T24细胞中受到抑制，但在共培养的HUVEC中被激活。在常氧性TME中，OXPHOS负责能量代谢。在HUVECs支持下，T24细胞显示出增加的增殖和迁移。在低氧TME中，糖酵解可能是主要的能量供应途径。然后，T24细胞表现出抑制的增殖和迁移，而HUVEC倾向于促进血管生成以适应恶劣的TME。OXPHOS负责能量代谢。在HUVECs支持下，T24细胞显示出增加的增殖和迁移。在低氧TME中，糖酵解可能是主要的能量供应途径。然后，T24细胞表现出抑制的增殖和迁移，而HUVEC倾向于促进血管生成以适应恶劣的TME。OXPHOS负责能量代谢。在HUVECs支持下，T24细胞显示出增加的增殖和迁移。在低氧TME中，糖酵解可能是主要的能量供应途径。然后，T24细胞表现出抑制的增殖和迁移，而HUVEC倾向于促进血管生成以适应恶劣的TME。