We present software-based methods for automatic phase control and for mosaicing high-speed, Lissajous-scanned images. To achieve imaging speeds fast enough for mosaicing, we first increase the image update rate tenfold from 3 to 30 Hz, then vertically interpolate each sparse image in real-time to eliminate fixed pattern noise. We validate our methods by imaging fluorescent beads and automatically maintaining phase control over the course of one hour. We then image fixed mouse brain tissues at varying update rates and compare the resulting mosaics. Using reconstructed image data as feedback for phase control eliminates the need for phase sensors and feedback controllers, enabling long-term imaging experiments without additional hardware. Mosaicing subsampled images results in videorate imaging speeds, nearly fully recovered spatial resolution, and millimeter-scale fields of view.

中文翻译：

---

使用 Lissajous 扫描共聚焦显微镜的基于软件的相位控制、视频速率成像和实时拼接。

我们提出了基于软件的自动相位控制方法和高速、Lissajous 扫描图像的拼接方法。为了获得足够快的成像速度以进行拼接，我们首先将图像更新速率从 3 Hz 提高到 30 Hz 十倍，然后实时垂直插值每个稀疏图像以消除固定模式噪声。我们通过对荧光珠进行成像并在一小时内自动保持相位控制来验证我们的方法。然后，我们以不同的更新速率对固定的小鼠脑组织进行成像，并比较生成的马赛克。使用重建的图像数据作为相位控制的反馈消除了对相位传感器和反馈控制器的需求，无需额外硬件即可进行长期成像实验。镶嵌二次采样图像可提高视频速率成像速度，几乎完全恢复空间分辨率，