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Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays.
Environmental and Molecular Mutagenesis ( IF 2.8 ) Pub Date : 2019-09-06 , DOI: 10.1002/em.22332
Érima Maria de Amorim 1, 2 , Samuel Lima de Santana 2 , André Severino da Silva 2 , Nayara Coriolano de Aquino 3 , Edilberto Rocha Silveira 3 , Rafael Matos Ximenes 1 , Claudia Rohde 2
Affiliation  

Medicinal plants are worldwide used as an efficient treatment of many diseases. Myracrodruon urundeuva Allemão (Anacardiaceae) is widely used Brazilian folk medicine to treat inflammations and infections of the female genital tract, conditions of the stomach and throat, and to heal wounds on the skin and mucous membranes. Several pharmacological properties of extracts and compounds isolated from M. urundeuva are found in the literature, corroborating its uses as antiulcer and gastroprotective, anti-inflammatory and analgesic, as well as antimicrobial. Despite these many uses in traditional herbal medicine, there are few reports of its toxic-genetic effect. This work aimed to investigate the genotoxic and mutagenic potential in vivo of the dry decoction of M. urundeuva leaves on somatic cells of Drosophila melanogaster, through the Comet assay and somatic mutation and recombination test (SMART). Six concentrations (0.5, 1.0, 2.0, 4.0, 8.0, and 16.0 mg/mL) were studied after feeding individuals for 24 hr in culture medium hydrated with extracts of M. urundeuva. In the Comet assay, all concentrations showed a genotoxic effect significantly higher than the negative control group, treated with distilled water. The two highest concentrations were also superior to the positive control group, treated with cyclophosphamide (1 mg/mL). In the SMART, there was a mutagenic effect at all concentrations tested, with a clear dose-dependent relationship. Both recombination and mutation account for these mutagenic effects. The set of results indicate that the dry decoction of M. urundeuva leaves is genotoxic and mutagenic for D. melanogaster under the experimental conditions of this study. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

中文翻译:

通过彗星和SMART分析评估黑腹果蝇体细胞中的Myracrodruon urundeuvaAllemão(Anacardiaceae)叶片干煎剂的遗传毒性。

药用植物在世界范围内被用作许多疾病的有效治疗方法。Myracrodruon urundeuvaAllemão(Anacardiaceae)被广泛用于巴西民间医学,用于治疗女性生殖道的炎症和感染,胃和喉咙的状况以及治愈皮肤和粘膜上的伤口。从文献中发现了从乌氏支原体分离出的提取物和化合物的几种药理特性,证实了其作为抗溃疡和胃保护,抗炎和镇痛以及抗菌剂的用途。尽管在传统草药中有许多用途,但鲜有关于其毒性遗传作用的报道。这项工作旨在研究黑果果蝇叶片干果蝇对果蝇果蝇体细胞的遗传毒性和诱变潜力,通过彗星分析和体细胞突变与重组测试(SMART)。将个体喂入水化莫氏支原体提取物的培养基中24小时后,研究了六种浓度(0.5、1.0、2.0、4.0、8.0和16.0 mg / mL)。在彗星试验中,所有浓度的遗传毒性作用均显着高于用蒸馏水处理的阴性对照组。最高的两个浓度也优于用环磷酰胺(1 mg / mL)处理的阳性对照组。在SMART中,在所有测试浓度下均具有诱变作用,并具有明显的剂量依赖性关系。重组和突变都解释了这些诱变作用。这组结果表明,M。urundeuva叶片的干汤对D具有遗传毒性和致突变性。黑色素瘤在本研究的实验条件下。环境。大声笑 诱变剂。2019.©2019 Wiley Periodicals,Inc.
更新日期:2020-03-20
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