BACKGROUND Aberrant expression of circular RNAs (circRNAs) is implicated in tumorigenesis and disease progression. However, the underlying molecular mechanisms and physiological functions of hsa_circ_0005075 in human colorectal cancer are still poorly understood. METHODS Quantitative real-time PCR (qRT-PCR) was performed to detect the expression level of hsa_circ_0005075 in colorectal cancer. Correlations of hsa_circ_0005075 expression with pathological parameters and overall survival were assessed. CCK-8 and Transwell invasion assays were utilized to determine the effect of hsa_circ_0005075 on proliferation and invasion of colorectal cancer cells. RESULTS Hsa_circ_0005075 was overexpressed in colorectal cancer tissues compared with paracancerous tissues and intestinal polyps. Its expression level was associated with distal metastasis, invasion, tumor node metastasis stage, and tumor diameter in colorectal cancer, and was negatively correlated with overall survival of patients with colorectal cancer. Moreover, enforced expression of hsa_circ_0005075 potentiated the proliferation and invasive behavior of colorectal cancer cells. CONCLUSIONS Our findings suggested that hsa_circ_0005075 expression was increased in colorectal cancer and might serve as a promising diagnostic mark and therapy target of colorectal cancer.

中文翻译：

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Hsa_circ_0005075 促进结直肠癌细胞的增殖和侵袭。

背景技术环状RNA（circRNA）的异常表达与肿瘤发生和疾病进展有关。然而，hsa_circ_0005075在人类结直肠癌中的潜在分子机制和生理功能仍然知之甚少。方法采用实时定量PCR（qRT-PCR）检测hsa_circ_0005075在结直肠癌中的表达水平。评估了 hsa_circ_0005075 表达与病理参数和总生存期的相关性。CCK-8 和 Transwell 侵袭试验用于确定 hsa_circ_0005075 对结直肠癌细胞增殖和侵袭的影响。结果与癌旁组织和肠息肉相比，Hsa_circ_0005075 在结直肠癌组织中过表达。其表达水平与远端转移、侵袭、结直肠癌的肿瘤淋巴结转移分期、肿瘤直径与结直肠癌患者的总生存期呈负相关。此外，hsa_circ_0005075 的强制表达增强了结直肠癌细胞的增殖和侵袭行为。结论 我们的研究结果表明，hsa_circ_0005075 在结直肠癌中的表达增加，可能作为结直肠癌的一个有前途的诊断标志和治疗靶点。