Poxviruses are large DNA viruses that include the causal agent of human smallpox and vaccinia virus. Poxviruses replicate in cytoplasmic foci known as DNA factories. Here we show that a virus-encoded transcription factor, viral mRNA, cellular RNA-binding protein heterodimer G3BP/Caprin-1 (p137), translation initiation factors eIF4E and eIF4G, and ribosomal proteins are concentrated in the same subdomains of cytoplasmic DNA factories. Furthermore, a cell coinfected with two recombinant vaccinia viruses expressing a virus core protein fused to cyan or yellow fluorescent protein displayed separate cyan and yellow factories, indicating that each factory formed from a single genome and was the site of transcription and translation as well as DNA replication. Hijacking of the host translation apparatus within the factory likely enhances the efficiency of virus replication and contributes to the suppression of host protein synthesis, thereby facilitating poxvirus subjugation of the cell.

中文翻译：

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细胞质痘病毒工厂内转录和翻译的共定位协调病毒表达并抑制宿主功能。

痘病毒是大型 DNA 病毒，包括人类天花和痘苗病毒的病原体。痘病毒在称为 DNA 工厂的细胞质病灶中复制。在这里，我们展示了病毒编码的转录因子、病毒 mRNA、细胞 RNA 结合蛋白异二聚体 G3BP/Caprin-1 (p137)、翻译起始因子 eIF4E 和 eIF4G 以及核糖体蛋白集中在细胞质 DNA 工厂的相同子域中。此外，与表达与青色或黄色荧光蛋白融合的病毒核心蛋白的两种重组痘苗病毒共感染的细胞显示出分离的青色和黄色工厂，表明每个工厂由单个基因组形成，是转录和翻译以及 DNA 的位点。复制。