当前位置: X-MOL 学术Artif. Cells Nanomed. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Belinostat suppresses cell proliferation by inactivating Wnt/β-catenin pathway and promotes apoptosis through regulating PKC pathway in breast cancer.
Artificial Cells, Nanomedicine, and Biotechnology ( IF 5.8 ) Pub Date : 2019-12-01 , DOI: 10.1080/21691401.2019.1671855
Pengwei Lu 1 , Yuanting Gu 1 , Lin Li 1 , Fang Wang 1 , Xue Yang 1 , Yunqing Yang 1
Affiliation  

Belinostat is a histone deacetylase inhibitor drug capable of regulating cell growth in diverse cancers. Nonetheless, little information clarified the role of Belinostat in breast cancer. Hence, the functions of Belinostat in breast cancer cells survival was disclosed in this study. Belinostat at 50 and 100 μM were applied to manage MCF-7 cells, cell viability, Ki67 positive cells, cell cycle and apoptosis were monitored via MTT, immunohistochemistry and flow cytometry. Furthermore, the apoptosis-related factors, Wnt/β-catenin pathway and PKC pathway were tested through western blot and qRT-PCR. Lastly, in vivo effect of Belinostat was determined by a murine model. The results showed that Belinostat dampened cell viability, decreased the proportion of Ki67 positive cells and arrested cells at G0/G1 phase. The decreases of Wnt/β-catenin, CCND2 and Myc were observed in MCF-7 cells after Belinostat stimulation. Additionally, Belinostat induced cell apoptosis, meanwhile dampened Bcl-2 and raised Bax and Cleaved caspase 3 in a dose and time-dependent manner. Additionally, Belinostat activated PKC pathway by upgrading PKCδ and P53 expressions. Furthermore, Belinostat restrained tumour weight and volume in vivo. In summary, this study depicted that Belinostat prohibited proliferation and evoked apoptosis via mediating Wnt/β-catenin and PKC pathways in MCF-7 cells.

中文翻译:

Belinostat通过灭活Wnt /β-catenin途径抑制细胞增殖,并通过调节乳腺癌中的PKC途径促进细胞凋亡。

Belinostat是一种组蛋白脱乙酰基酶抑制剂药物,能够调节多种癌症中的细胞生长。但是,几乎没有信息澄清Belinostat在乳腺癌中的作用。因此,这项研究揭示了Belinostat在乳腺癌细胞存活中的功能。使用50和100μM的Belinostat处理MCF-7细胞,通过MTT,免疫组织化学和流式细胞仪监测细胞活力,Ki67阳性细胞,细胞周期和凋亡。此外,通过western blot和qRT-PCR检测凋亡相关因子,Wnt /β-catenin途径和PKC途径。最后,通过鼠模型确定了Belinostat的体内作用。结果表明,Belinostat抑制了细胞活力,降低了Ki0阳性细胞和G0 / G1期停滞细胞的比例。Wnt /β-catenin的减少 Belinostat刺激后在MCF-7细胞中观察到CCND2和Myc。此外,Belinostat诱导细胞凋亡,同时以剂量和时间依赖性方式抑制Bcl-2并升高Bax和Cleaved caspase 3。此外,Belinostat通过提高PKCδ和P53表达来激活PKC途径。此外,Belinostat在体内限制了肿瘤的重量和体积。总而言之,这项研究描述了Belinostat通过介导MCF-7细胞中的Wnt /β-catenin和PKC途径来禁止增殖并诱发凋亡。Belinostat可抑制体内的肿瘤重量和体积。总而言之,这项研究描述了Belinostat通过介导MCF-7细胞中的Wnt /β-catenin和PKC途径来禁止增殖并诱发凋亡。Belinostat可抑制体内的肿瘤重量和体积。总而言之,这项研究描述了Belinostat通过介导MCF-7细胞中的Wnt /β-catenin和PKC途径来禁止增殖并诱发凋亡。
更新日期:2019-11-01
down
wechat
bug