Due to the presence of the exoskeleton, the moult cycle is a required event in the life of crustaceans. In order for the exoskeleton to be replaced, it is necessary for these animals to uptake water from the environment for their body tissues during the late pre-moult, ecdysis and in the early post-moult for the expansion of the new cuticle. The mechanisms and organs used to uptake water in these events are not yet completely clear. In this study, we investigated the participation of aquaporins and Na+/K+-ATPase in cells of two potential organs responsible for the uptake of water (gills and gut) at three different stages of the moult cycle in freshwater shrimp Palaemon argentinus. We showed the participation of these two proteins with different functional patterns in gills and intestinal cells as water uptake pathways for moult and early post-moult. Our results indicate that Na+/K+-ATPase promotes the necessary osmotic gradient in the gills for water uptake through the gut cells during the pre-moult. This process, in turn, remains active during the post-moult stage with the addition of water influx through the gill cells.

中文翻译：

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Na+/K+-ATPase 和水通道蛋白在虾的换羽过程中对水的吸收作用 (Nobili, 1901)

由于外骨骼的存在，换羽周期是甲壳类动物生命中必不可少的事件。为了更换外骨骼，这些动物有必要在蜕皮前后期、蜕皮后期和蜕皮后早期从环境中吸收水分，以扩展新的角质层。在这些事件中用于吸收水分的机制和器官尚不完全清楚。在这项研究中，我们研究了水通道蛋白和 Na+/K+-ATP 酶在淡水虾阿根廷青虾蜕皮周期的三个不同阶段负责吸收水分的两个潜在器官（鳃和肠道）的细胞中的作用。我们展示了这两种具有不同功能模式的蛋白质在鳃和肠细胞中作为换羽和换羽后早期吸水途径的参与。我们的结果表明，Na+/K+-ATPase 促进了鳃中必要的渗透梯度，以便在换羽前通过肠道细胞吸收水分。反过来，这个过程在蜕皮后阶段保持活跃，并通过鳃细胞增加水流入。