SummaryThe effect of different dilution rates and equilibration times on the cryopreservation of Bactrian camel spermatozoa was evaluated in the current study. Semen samples from four healthy adult males were collected, processed and pooled. They were then subjected to a completely randomized 4×2 factorial design including four dilution rates (DR; 1:1, 1:2, 1:4 or 1:8; v:v with SHOTOR diluent) and two equilibration times (ET; 1 or 2 h at 5ºC). After freezing and thawing, sperm kinematic parameters as well as viability, plasma membrane integrity, abnormality and seminal malondialdehyde level were assessed. According to the results, four-fold diluted samples recorded significantly higher values (P < 0.05) for sperm total (39.58 vs 31.83 and 33.33,%) and progressive motility (19.50 vs 14.00 and 14.25,%), viability (55.37 vs 43.50 and 48.75,%) and plasma membrane integrity (46.75 vs 37.25 and 37.37,%) than those of both less (1:1) and high (1:8) concentrated samples, respectively. By contrast, the percentage of abnormal spermatozoa and the concentration of seminal malondialdehyde were comparable among all treated groups. Moreover, ET revealed that 1 h equilibration had significantly higher sperm motility (37.04 vs 33.33%), linearity (42.29 vs 32.26%), beat cross-frequency (13.15 vs 8.70 Hz), plasma membrane integrity (42.25 vs 39.75%) and viability (51.37 vs 48.12%) compared with 2 h of ET (P < 0.05). Taken together, a four-fold dilution along with 1 h equilibration can be an optimal procedure to cryopreserve Bactrian camel sperm.

中文翻译：

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使用不同稀释率和平衡时间优化双峰驼精子冷冻性

摘要本研究评估了不同稀释率和平衡时间对双峰骆驼精子冷冻保存的影响。收集、处理和汇集来自四名健康成年男性的精液样本。然后对它们进行完全随机的 4×2 因子设计，包括四种稀释率（DR；1:1、1:2、1:4 或 1:8；使用 SHOTOR 稀释剂的 v:v）和两个平衡时间（ET； 5ºC 下 1 或 2 小时）。冷冻和解冻后，评估精子运动学参数以及活力、质膜完整性、异常和精液丙二醛水平。根据结果​​，四倍稀释的样品记录的值明显更高（磷< 0.05) 精子总数 (39.58 vs 31.83 和 33.33,%) 和进行性运动 (19.50 vs 14.00 和 14.25,%)、活力 (55.37 vs 43.50 和 48.75,%) 和质膜完整性 (46.75 vs 37.25 和 37.37,% ) 分别低于浓度较低 (1:1) 和高 (1:8) 的样本。相比之下，异常精子的百分比和精液丙二醛的浓度在所有治疗组中相当。此外，ET 显示 1 小时平衡具有显着更高的精子活力（37.04 对 33.33%）、线性（42.29 对 32.26%）、跳动交叉频率（13.15 对 8.70 赫兹）、质膜完整性（42.25 对 39.75%）和活力(51.37 vs 48.12%) 与 2 小时 ET (磷< 0.05)。总之，四倍稀释和 1 小时平衡可能是低温保存双峰骆驼精子的最佳程序。