Background: The pharmacological application of kaempferol, a natural flavonol present in different plant species, has been demonstrated to have extensive anti-inflammatory, anti-apoptotic, anti-oxidative, and anti-cancer effects. Pyroptosis is an inflammatory form of programed cell death by membranolysis and associated leakage of cytoplasm. This study investigated the molecular mechanism of kaempferol-induced effects on the pyroptosis in splenic lymphocytes (SLCs) isolated from mice. Methods: Lipopolysaccharide (LPS)-primed and adenosine triphosphate (ATP)-stimulated SLCs were used to establish the pyroptosis model. The kaempferol pretreatment was tested in the model. Results: The results show that kaempferol alleviates LPS-ATP mediated damage by increasing cell viability, improving membrane integrity, and decreasing the release of IL1b and IL-18. Kaempferol reduces pyroptosis by suppressing the expression and activity of caspase-1, increasing the protein expression of Toll-like receptor 4 (TLR4) and NOD-like receptor 3 (NLRP3), and inhibition of the decomposition of gasdermin D (GSDMD). Conclusions: Our data suggest that kaempferol exhibits anti-pyroptosis activities, which warrants further detailed investigation.

中文翻译：

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山emp酚可通过调节小鼠的热凋亡途径减轻LPS-ATP介导的脾脏淋巴细胞炎症性损伤。

背景：山emp酚（一种存在于不同植物物种中的天然黄酮醇）的药理作用已被证明具有广泛的抗炎，抗凋亡，抗氧化和抗癌作用。细胞凋亡是膜分解和相关细胞质渗漏导致程序性细胞死亡的炎性形式。这项研究调查了山萘酚对小鼠脾脏淋巴细胞（SLCs）中的细胞凋亡的影响的分子机制。方法：使用脂多糖（LPS）引发的三磷酸腺苷（ATP）刺激的SLC建立发烧模型。在模型中测试了山萘酚预处理。结果：结果表明山emp酚可通过增加细胞活力，改善膜完整性并减少IL1b和IL-18的释放来减轻LPS-ATP介导的损伤。山emp酚可通过抑制caspase-1的表达和活性，增加Toll样受体4（TLR4）和NOD样受体3（NLRP3）的蛋白表达以及抑制胃泌素D（GSDMD）的分解来减少细胞凋亡。结论：我们的数据表明山奈酚具有抗化脓活性，值得进一步详细研究。