Genetic diversity of Indo-China rice varieties using ISSR, SRAP and InDel markers.,Journal of Genetics

当前位置： X-MOL 学术 › J. Genet. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Genetic diversity of Indo-China rice varieties using ISSR, SRAP and InDel markers.
Journal of Genetics ( IF 1.5 ) Pub Date : 2019-09-24
Pattaraborn Moonsap ₁ , Nutthalak Laksanavilat , Sittipun Sinumporn , Piyama Tasanasuwan , Sureeporn Kate-Ngam , Chatchawan Jantasuriyarat

Affiliation

Rice is believed to have originated from Indo-China, area between China and India, and then spread throughout the world. The Indochina region mainly includes countries like Thailand, Laos and Vietnam, which are the world's major rice exporters. Rice varieties grown in this area are highly diverse due to their different environment, ecosystem and climatic conditions. The objective of this study was to evaluate the genetic relationship of Indochina rice varieties using intersimple sequence repeat (ISSR), sequence-related amplified polymorphism (SRAP) and insertion-deletion (InDel) markers. Forty-six rice varieties, including 16, 4,11 and 15 from Thailand, China, Laos and Vietnam, respectively were used in this study. Seventeen of the 20 ISSR primers showed 82.96% polymorphism. At the same time, 17 of the 30 primer pairs of SRAP marker showed clear DNA amplification, which resulted in 84.79% polymorphism. Ninety-seven of 133 InDel markers have about 99.47% polymorphism. Three markers showed average PIC score ranging from 0.20 to 0.26. When the analysis was conducted using UPGMA clustering method, it was found that the combined data from three markers gave a better result than each marker separately. The results from clustering analysis showed that all accessions can be grouped based on their location and can be categorized into two major groups. Useful results from this study could bring substantial benefits and ultimately help the rice breeders to develop elite rice varieties in future.

更新日期：2019-11-01

点击分享

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>