Genomic analysis of circulating, cell-free DNA (cfDNA) is being used extensively for molecular diagnostics. Many approaches rely on the construction of cfDNA genomic libraries, targeted retrieval of specific genomic regions and analysis by next-generation DNA sequencing. Several steps during sample preparation require isolation of DNA fragments within a particular size range. In this Benchmark article, two related methods for size-selective DNA fragment enrichment are described.

中文翻译：

---

针对依赖下一代测序的液体活检应用，集中选择无细胞DNA样品的大小。

循环无细胞DNA（cfDNA）的基因组分析已广泛用于分子诊断。许多方法都依赖于cfDNA基因组文库的构建，特定基因组区域的目标检索以及下一代DNA测序的分析。样品制备过程中的几个步骤要求分离特定大小范围内的DNA片段。在这篇基准文章中，描述了两种相关的大小选择DNA片段富集方法。