The extra-hospital epidemiology of Acinetobacter infections is a subject of debate. In recent years, the prevalence of animal multidrug-resistant Acinetobacter infections has increased considerably. The goal of the present study was to specify Acinetobacter species isolated from laboratory mice and to test them for their antimicrobial susceptibility. During routine microbiological monitoring of laboratory mice, 12 Acinetobacter spp. were isolated. By means of 16S rRNA and rpoB gene sequencing, seven of the isolates were identified as Acinetobacter radioresistens, three isolates belonged to Acinetobacter genomospecies 14BJ, one isolate was classified as Acinetobacter pitii and one as Acinetobacter sp. ANC 4051. The distribution of the minimal inhibitory concentration (MIC) values was uniform for 21 of the 23 antimicrobial agents tested, whereas a broad MIC distribution was recorded for tulathromycin and streptomycin. The MIC values recorded were low for the majority of the antibiotics tested. Nevertheless, very high MIC values, which will probably render a therapeutic approach using these substances unsuccessful, were recorded for florfenicol, tiamulin, tilmicosin and cephalothin in most of the isolates. In conclusion, we document colonization of laboratory mice with different Acinetobacter species, displaying similar antibiotic susceptibility profiles, with possible implications in the Acinetobacter epidemiology as well as in the husbandry and experimentation of the colonized animals.

中文翻译：

---

实验小鼠中的不动杆菌属：物种调查和抗菌素耐药性。

不动杆菌感染的院外流行病学是一个争论的话题。近年来，动物多药耐药性不动杆菌感染的患病率已大大提高。本研究的目的是确定从实验室小鼠中分离出的不动杆菌种类，并对其抗菌性进行测试。在实验室小鼠的常规微生物监测过程中，不动杆菌属为12个。被隔离了。通过16S rRNA和rpoB基因测序，鉴定出7株为不动杆菌，其中3株属于不动杆菌基因14BJ，1株为皮氏不动杆菌，1株为不动杆菌。ANC4051。在测试的23种抗菌剂中，有21种的最小抑菌浓度（MIC）分布均匀，而图拉霉素和链霉素的MIC分布较广。对于大多数测试的抗生素，记录的MIC值均较低。然而，在大多数分离物中，氟苯尼考，提洛米林，替米考星和头孢菌素仍记录到非常高的MIC值，这可能会使使用这些物质的治疗方法不成功。总之，我们记录了具有不同不动杆菌属物种的实验小鼠的定殖，显示出相似的抗生素敏感性分布，可能对不动杆菌的流行病学以及在定殖的动物的饲养和实验中产生影响。多数分离物中记录有氟苯尼考，替米林，替米考星和头孢菌素可能无法成功使用这些物质进行治疗。总之，我们记录了具有不同不动杆菌属物种的实验小鼠的定殖，显示出相似的抗生素敏感性分布，可能对不动杆菌的流行病学以及在定殖的动物的饲养和实验中产生影响。多数分离物中记录有氟苯尼考，替米林，替米考星和头孢菌素可能无法成功使用这些物质进行治疗。总之，我们记录了具有不同不动杆菌属物种的实验小鼠的定殖，显示出相似的抗生素敏感性分布，可能对不动杆菌的流行病学以及在定殖的动物的饲养和实验中产生影响。