ABSTRACT The aim of the study was to investigate the effects of dietary alpha-ketoglutarate (AKG) on the faecal bacteria composition of suckling piglets after supplementation of AKG to the diet of lactating sows. After farrowing, the sows were assigned to either a normal lactation diet (control group, n = 12) or a diet supplemented with 0.25% AKG (AKG group, n = 12) based on body weight (BW) and parity. During the 21-d suckling period, BW and diarrhoea occurrences of piglets were recorded daily, while faeces were sampled weekly from sows and piglets. The levels of pH, ammonia, short-chain fatty acids (SCFA) and lactate in the faeces of piglets were determined. In particular, bacteria profiles in faeces of sows and their suckling piglets were examined by Illumina sequencing. The results showed that the AKG diet altered the faecal bacteria composition in sows during the 21-d lactation period, leading to increases (p < 0.05) in the abundances of genera Prevotella, Lactobacillus, Bacteroides and Methanobrevibacter, but decreases (p < 0.05) in the abundances of genera Oscillospira and Dorea. AKG supplement to the sows during lactation indirectly enhanced (p < 0.05) bacterial richness and SCFA levels (especially, acetate) in the faeces of piglets during the 21-d suckling period. It is suggested that maternal AKG supplementation alters the composition of faecal bacteria in the sows, and increases the faecal bacteria richness and acetate levels in the piglets, which might be associated with an enhanced growth performance of piglets.

中文翻译：

---

日粮α-酮戊二酸对泌乳母猪及其哺乳仔猪粪便中细菌分布的影响

摘要 本研究的目的是研究日粮中α-酮戊二酸（AKG）对泌乳母猪日粮中添加AKG后乳仔猪粪便细菌组成的影响。分娩后，根据体重 (BW) 和胎次，将母猪分配到正常泌乳日粮（对照组，n = 12）或补充 0.25% AKG 的日粮（AKG 组，n = 12）。在 21 天的哺乳期，每天记录仔猪的体重和腹泻发生率，同时每​​周从母猪和仔猪身上取样。测定了仔猪粪便中的 pH、氨、短链脂肪酸 (SCFA) 和乳酸水平。特别是，通过 Illumina 测序检查了母猪及其哺乳仔猪粪便中的细菌谱。结果表明，AKG 日粮改变了母猪在 21 天泌乳期的粪便细菌组成，导致普氏菌属、乳杆菌属、拟杆菌属和甲烷短杆菌属的丰度增加（p < 0.05），但减少（p < 0.05）在 Oscillospira 和 Dorea 属的丰度中。在哺乳期间向母猪补充 AKG 间接提高了（p < 0.05）仔猪在 21 天哺乳期粪便中的细菌丰富度和 SCFA 水平（尤其是醋酸盐）。建议母猪补充AKG改变母猪粪便细菌的组成，增加仔猪粪便细菌的丰富度和醋酸盐水平，这可能与仔猪生长性能的提高有关。05) 在普雷沃氏菌属、乳杆菌属、拟杆菌属和甲烷短杆菌属的丰度中增加，但在响螺菌属和多雷亚菌属的丰度中降低 (p < 0.05)。在哺乳期间向母猪补充 AKG 间接提高了（p < 0.05）仔猪在 21 天哺乳期粪便中的细菌丰富度和 SCFA 水平（尤其是醋酸盐）。建议母猪补充AKG改变母猪粪便细菌的组成，增加仔猪粪便细菌的丰富度和醋酸盐水平，这可能与仔猪生长性能的提高有关。05) 在普雷沃氏菌属、乳杆菌属、拟杆菌属和甲烷短杆菌属的丰度中增加，但在响螺菌属和多雷亚菌属的丰度中降低 (p < 0.05)。在哺乳期间向母猪补充 AKG 间接提高了（p < 0.05）仔猪在 21 天哺乳期粪便中的细菌丰富度和 SCFA 水平（尤其是醋酸盐）。建议母猪补充AKG改变母猪粪便细菌的组成，增加仔猪粪便细菌的丰富度和醋酸盐水平，这可能与仔猪生长性能的提高有关。0.05) 21 天哺乳期仔猪粪便中的细菌丰富度和 SCFA 水平（尤其是醋酸盐）。建议母猪补充AKG改变母猪粪便细菌的组成，增加仔猪粪便细菌的丰富度和醋酸盐水平，这可能与仔猪生长性能的提高有关。0.05) 21 天哺乳期仔猪粪便中的细菌丰富度和 SCFA 水平（尤其是醋酸盐）。建议母猪补充AKG改变母猪粪便细菌的组成，增加仔猪粪便细菌的丰富度和醋酸盐水平，这可能与仔猪生长性能的提高有关。