Long non-coding RNAs (lncRNA) exert biological functions by interacting with RNAs, proteins, and DNA. Although lung damage associated with radon exposure was attributed to disturbances in microRNA and protein expression, the influence of radon on lncRNA is at present not known. The aim of this study was to (1) examine the effect of radon on lncRNA-mediated expression of transcription factors in mRNA in mouse lung tissue and (2) determine potential function and targets. Female BALB/c mice were divided into two groups: control and radon exposure to approximately 100,000 Bq/m3 (equivalent up to 60 working level month, WLM).RNA was extracted from lung tissue and used for high through-put lncRNA microarray analysis. A total of 1256 lncRNA transcripts were differentially expressed between the two groups of mice. Among these, the top 200 lncRNA-mRNA sets, with fold change of >2 and p-value <.05, were selected for KEGG analysis. Functional analysis via bioinformatics prediction in this study also suggested involvement of ErbB and Notch pathways in radon-induced mouse pulmonary injury. The results from immunohistochemical and Western blot analysis indicated that EbB2 and k-Ras protein expressions were significantly increased. In conclusion, approximately 1,000 dysregulated lncRNA transcripts were found in radon-exposed mice and these lncRNA may play an important role in lung damage following radon exposure. The observations in this study also suggested that ErbB2 and Notch pathways are activated and may be involved in radon-induced pulmonary toxicity.

中文翻译：

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长non非编码RNA在暴露于long的小鼠肺组织中的表达特征。

长非编码RNA（lncRNA）通过与RNA，蛋白质和DNA相互作用发挥生物学功能。尽管与ra暴露相关的肺损伤归因于微小RNA和蛋白质表达的紊乱，但目前尚不知道of对lncRNA的影响。这项研究的目的是（1）研究ra对小鼠肺组织mRNA中lncRNA介导的转录因子表达的影响，以及（2）确定潜在的功能和靶标。将雌性BALB / c小鼠分为两组：对照组和ra暴露于大约100,000 Bq / m3（相当于最高60个工作水平月，WLM）。从肺组织中提取RNA并用于高通量lncRNA微阵列分析。两组小鼠之间总共差异表达了1256个lncRNA转录物。其中，前200个lncRNA-mRNA集，倍数变化> 2和p值<.05的样本被选择用于KEGG分析。通过这项研究中的生物信息学预测进行的功能分析还表明，ErbB和Notch通路参与了ra诱导的小鼠肺损伤。免疫组织化学和蛋白质印迹分析的结果表明，EbB2和k-Ras蛋白表达显着增加。总之，在暴露于ra的小鼠中发现了约1,000个失调的lncRNA转录物，这些lncRNA可能在暴露于don的肺损伤中起重要作用。在这项研究中的观察结果还表明，ErbB2和Notch途径被激活，可能参与ra诱导的肺毒性。通过这项研究中的生物信息学预测进行的功能分析还表明，ErbB和Notch通路参与了ra诱导的小鼠肺损伤。免疫组织化学和蛋白质印迹分析的结果表明，EbB2和k-Ras蛋白表达显着增加。总之，在暴露于ra的小鼠中发现了约1,000个失调的lncRNA转录物，这些lncRNA可能在暴露于don的肺损伤中起重要作用。在这项研究中的观察结果还表明，ErbB2和Notch途径被激活，可能参与ra诱导的肺毒性。通过这项研究中的生物信息学预测进行的功能分析还表明，ErbB和Notch通路参与了ra诱导的小鼠肺损伤。免疫组织化学和蛋白质印迹分析的结果表明，EbB2和k-Ras蛋白表达显着增加。总之，在暴露于ra的小鼠中发现了约1,000个失调的lncRNA转录物，这些lncRNA可能在暴露于don的肺损伤中起重要作用。在这项研究中的观察结果还表明，ErbB2和Notch途径被激活，可能参与ra诱导的肺毒性。免疫组织化学和蛋白质印迹分析的结果表明，EbB2和k-Ras蛋白表达显着增加。总之，在暴露于mice的小鼠中发现了约1,000个失调的lncRNA转录物，这些lncRNA可能在暴露于don的肺损伤中起重要作用。在这项研究中的观察结果还表明，ErbB2和Notch途径被激活，可能参与ra诱导的肺毒性。免疫组织化学和蛋白质印迹分析的结果表明，EbB2和k-Ras蛋白表达显着增加。总之，在暴露于mice的小鼠中发现了约1,000个失调的lncRNA转录物，这些lncRNA可能在暴露于don的肺损伤中起重要作用。在这项研究中的观察结果还表明，ErbB2和Notch途径被激活，可能参与ra诱导的肺毒性。