Uncoupler-adapted Euglena gracilis have a greatly impaired capacity to take up and incorporate some exogenously supplied amino acids and sugars. The degree of inhibition varies widely from >90% in the case of valine or glucose to none in the case of histidine. The inhibition is due to a decreased activity of the transport mechanism itself and is not due to either a lesion in the control mechanism for endogenous amino acid or sugar synthesis nor to a direct inhibition of the transport mechanism by uncouplers. No preferential labeling of mitochondrial membranes by [14C]amino acids occurs during the process of adaptation, a time when no cell division occurs. Apparently, during the long time required for adaptation, there occurs no major modification of mitochondrial proteins which could explain the subsequent resistance to uncouplers.

中文翻译：

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适应解偶联剂的 Euglena gracilis 中氨基酸和糖转运的修饰。

适应解偶联剂的 Euglena gracilis 吸收和吸收一些外源性氨基酸和糖的能力受到极大损害。抑制程度从缬氨酸或葡萄糖的>90% 到组氨酸的无。这种抑制是由于转运机制本身的活性降低，而不是由于内源性氨基酸或糖合成的控制机制受损，也不是由于解偶联剂对转运机制的直接抑制。在不发生细胞分裂的适应过程中，[14C] 氨基酸不会优先标记线粒体膜。显然，在适应所需的长时间内，线粒体蛋白没有发生重大改变，这可以解释随后对解偶联剂的抗性。