Nanoparticles (NPs) in contact with biological fluids experience changes in surface chemistry that can impact their biodistribution and downstream physiological impact. One such change involves the formation of a protein corona (PC) on the surface of NPs. Here we present a foundational study on PC formation following the incubation of polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs, 50 nm) in the plasma of smallmouth bass (Micropterus dolomieu). PC formation increases with exposure time and is also affected by gender, with AgNPs incubated in male plasma having slightly thinner PCs and less negative zeta potentials than those incubated in female plasma. Proteomic analysis also revealed gender-specific differences in PC composition: in particular, egg-specific proteins (vitellogenin (VTG) and zona pellucida (ZP) were identified only in PCs derived from female plasma, raising the possibility of their roles in AgNP-related reproductive toxicity by promoting their accumulation in developing oocytes.

中文翻译：

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鱼血浆中银纳米颗粒的蛋白质电晕分析。

与生物流体接触的纳米颗粒（NP）会经历表面化学变化，从而影响其生物分布和下游生理影响。一种这样的变化涉及在NP表面上形成蛋白质电晕（PC）。在这里，我们介绍了在小口黑鲈（Micropterus dolomieu）血浆中孵育聚乙烯基吡咯烷酮涂层的AgNPs（PVP-AgNPs，50 nm）之后对PC形成的基础研究。PC形成随着暴露时间的增加而增加，并且也受到性别的影响，在雄性血浆中孵育的AgNP比在雌性血浆中孵育的AgNP具有更薄的PC和更低的zeta电位。蛋白质组学分析还揭示了PC成分的性别差异：特别是，