Introduction of more than one gene into crop plants simultaneously or sequentially, called transgene stacking, has been a more effective strategy for conferring higher and durable insect and disease resistance in transgenic plants than single-gene technology. Transgenes can be stacked against one or more pathogens or for traits such as herbicide tolerance or anthocyanin pigmentation. Polygenic agronomic traits can be improved by multiple gene transformation. The most widely engineered stacked traits are insect resistance and herbicide tolerance as these traits may lead to lesser use of pesticides, higher yield, and efficient control of weeds. In this review, we summarize transgene stacking of two or more transgenes into crops for different agronomic traits, potential applications of gene stacking, its limitations and future prospects.

中文翻译：

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转基因堆叠是增强植物抗病性的有效工具。

与单基因技术相比，向转基因植物同时或依次引入多个基因（称为转基因堆叠）是赋予转基因植物更高和持久的昆虫和疾病抗性的更有效策略。可以针对一种或多种病原体或针对诸如除草剂耐受性或花色苷色素沉着等性状堆叠转基因。多基因转化可以改善多基因农艺性状。工程最广泛的叠加性状是对昆虫的抗性和对除草剂的耐受性，因为这些性状可能导致减少使用农药，提高产量并有效控制杂草。在这篇综述中，我们总结了两种或更多种转基因进入农作物的转基因堆叠，它们具有不同的农艺性状，基因堆叠的潜在应用，其局限性和未来前景。