The Papez circuit is crucial for several brain functions, including long-term memory and emotion. Estradiol modulates cognitive functions based on the expression pattern of its receptor subtypes including estrogen receptor (ER) α, β, and G protein-coupled receptor 30 (GPR30). Similarly, the activity in the cholinergic system correlates with several brain functions, such as learning and memory. In this study, we used immunofluorescence to examine the expression patterns of ERβ and Western blotting to analyze GPR30 and choline acetyltransferase (ChAT) expression, in different regions of the Papez circuit, including the prefrontal cortex, hippocampus, hypothalamus, anterior nucleus of the thalamus, and cingulum in female rats at postnatal days (PND) 1, 10, and 56. Our main finding was that the highest expression of ERβ and GPR30 was noted in each brain area of the Papez circuit in the PND1 rats, whereas the expression of ChAT was the highest in PND10 rats. These results provide vital information on the postnatal expression patterns of ER subtypes and ChAT in different regions of the Papez circuit.

中文翻译：

---

帕皮兹环路不同区域的雌激素受体亚型和胆碱乙酰转移酶的产后表达模式。

Papez回路对于多种大脑功能（包括长期记忆和情感）至关重要。雌二醇基于其受体亚型的表达模式来调节认知功能，所述受体亚型包括雌激素受体（ER）α，β和G蛋白偶联受体30（GPR30）。同样，胆碱能系统的活动与几种脑功能有关，例如学习和记忆。在这项研究中，我们使用免疫荧光检查了ERβ的表达模式，并通过Western印迹分析了Papez回路不同区域（包括前额叶皮层，海马，下丘脑，丘脑前核）中GPR30和胆碱乙酰转移酶（ChAT）的表达。雌鼠在出生后第1天，第10天和第56天的，和扣带。我们的主要发现是，在PND1大鼠的Papez回路的每个大脑区域中，ERβ和GPR30的表达最高，而在PND10大鼠中，ChAT的表达最高。这些结果提供有关帕皮兹回路不同区域的ER亚型和ChAT的产后表达模式的重要信息。