ABSTRACT Single-cell chromosome conformation capture approaches are revealing the extent of cell-to-cell variability in the organization and packaging of genomes. These single-cell methods, unlike their multi-cell counterparts, allow straightforward computation of realistic chromosome conformations that may be compared and combined with other, independent, techniques to study 3D structure. Here we discuss how single-cell Hi-C and subsequent 3D genome structure determination allows comparison with data from microscopy. We then carry out a systematic evaluation of recently published single-cell Hi-C datasets to establish a computational approach for the evaluation of single-cell Hi-C protocols. We show that the calculation of genome structures provides a useful tool for assessing the quality of single-cell Hi-C data because it requires a self-consistent network of interactions, relating to the underlying 3D conformation, with few errors, as well as sufficient longer-range cis- and trans-chromosomal contacts.

中文翻译：

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用显微镜比较染色体构象捕获实验的 3D 基因组结构计算：单细胞 Hi-C 协议的评估。

摘要单细胞染色体构象捕获方法正在揭示基因组组织和包装中细胞间变异的程度。与多细胞方法不同，这些单细胞方法允许直接计算真实的染色体构象，这些构象可以与其他独立的技术进行比较和组合来研究 3D 结构。在这里，我们讨论单细胞 Hi-C 和随后的 3D 基因组结构测定如何与显微镜数据进行比较。然后，我们对最近发布的单细胞 Hi-C 数据集进行系统评估，以建立用于评估单细胞 Hi-C 协议的计算方法。