ABSTRACT Methods of super-resolving light microscopy (SRM) have found an exponentially growing range of applications in cell biology, including nuclear structure analyses. Recent developments have proven that Single Molecule Localization Microscopy (SMLM), a type of SRM, is particularly useful for enhanced spatial analysis of the cell nucleus due to its highest resolving capability combined with very specific fluorescent labeling. In this commentary we offer a brief review of the latest methodological development in the field of SMLM of chromatin designated DNA Structure Fluctuation Assisted Binding Activated Localization Microscopy (abbreviated as fBALM) as well as its potential future applications in biology and medicine.

中文翻译：

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通过 DNA 构象的可逆变化进行超分辨率结合激活定位显微镜

摘要 超分辨光学显微镜 (SRM) 方法在细胞生物学中的应用范围呈指数增长，包括核结构分析。最近的发展已经证明，单分子定位显微镜 (SMLM) 是一种 SRM，由于其最高的分辨率与非常特定的荧光标记相结合，因此对于增强细胞核的空间分析特别有用。在这篇评论中，我们简要回顾了染色质指定的 DNA 结构波动辅助结合激活定位显微镜（缩写为 fBALM）的 SMLM 领域的最新方法学发展及其在生物学和医学中的潜在应用。