当前位置:
X-MOL 学术
›
IEEE Trans. NanoBiosci.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
3D Printed Bioelectrodes for Enzymatic Biofuel Cell: Simple, Rapid, Optimized and Enhanced Approach.
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2019-09-13 , DOI: 10.1109/tnb.2019.2941196 Prakash Rewatkar , Sanket Goel
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2019-09-13 , DOI: 10.1109/tnb.2019.2941196 Prakash Rewatkar , Sanket Goel
Research to develop sustainable, automated and robust Enzymatic Biofuel Cell (EBFC) with high-energy output is hugely driven from its multiple application domains. To this end, continuous work to realize inexpensive and mass-manufacturable bioelectrodes has remained one of the prime challenges. In the present work, such bioelectrodes (both bioanode and biocathode) have been created by leveraging customized and novel 3D printed (3DP) composite Graphene/PLA filaments. The unsophisticated method is cost-effective and rapid, eradicates the requirement of any further amendment and post-processing treatment, and is amenable to print the end product within a short duration. To enhance the surface area and optimize the electrochemical sensing, the fabricated 3DP Graphene/PLA electrodes were treated with dimethylformamide (DMF) solution followed by the immobilization of GOx and laccase enzymes to realize bioanode and biocathode respectively. The morphological and elemental composition study of 3DP, 3DP/DMF treated and enzyme modified electrodes was carried out via scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) respectively. The electrochemical and polarization performances of the prepared bioelectrodes were evaluated using the linear sweep voltammetry (LSV), cyclic voltammetry (CV), and open circuit potential (OCP). Maximum current density of 1.41 mA/cm2 at 0.5 V for bioanode at 40 mM glucose concentration, and 0.216 mA/cm2 at 0.42 V was noticed for biocathode. To provide proof-of-concept, the fabricated bioelectrodes were assembled in a fluidic cell and the polarization performance was studied. Overall, the fabricated 3DP bioelectrode exhibited good stability with reasonably good retention of biocatalytic activity. The fabricated 3DP Composite Graphene/PLA electrodes are promising bioelectrodes material for EBFC and many electrochemical based biochemical sensing applications at the microfluidic level.
中文翻译:
用于酶促生物燃料电池的3D打印生物电极:简单,快速,优化和增强的方法。
开发具有高能量输出的可持续,自动化和强大的酶促生物燃料电池(EBFC)的研究受到其多个应用领域的极大推动。为此,实现廉价且可大量制造的生物电极的持续工作仍然是主要挑战之一。在当前的工作中,通过利用定制的和新颖的3D打印(3DP)复合石墨烯/ PLA细丝创建了此类生物电极(生物阳极和生物阴极)。这种简单的方法经济高效,快速,消除了任何进一步修改和后期处理的要求,并适合在短时间内印刷最终产品。为了增加表面积并优化电化学感应,用二甲基甲酰胺(DMF)溶液处理3DP石墨烯/ PLA电极,然后固定GOx和漆酶,分别实现生物阳极和生物阴极。分别通过扫描电子显微镜(SEM)和能量分散光谱(EDS)对3DP,3DP / DMF处理和酶修饰的电极进行了形态和元素组成研究。使用线性扫描伏安法(LSV),循环伏安法(CV)和开路电势(OCP)评估了制备的生物电极的电化学和极化性能。在40 mM葡萄糖浓度下,生物阳极在0.5 V时的最大电流密度为1.41 mA / cm2,在生物阴极中则为0.42 V时的最大电流密度为0.216 mA / cm2。为了提供概念证明,将制备的生物电极组装在流体池中并研究其极化性能。总的来说,所制造的3DP生物电极表现出良好的稳定性,并具有相当好的生物催化活性保留。所制造的3DP复合石墨烯/ PLA电极是有前途的EBFC生物电极材料,在许多微流控水平上都是基于电化学的生物化学传感应用。
更新日期:2019-11-01
中文翻译:
用于酶促生物燃料电池的3D打印生物电极:简单,快速,优化和增强的方法。
开发具有高能量输出的可持续,自动化和强大的酶促生物燃料电池(EBFC)的研究受到其多个应用领域的极大推动。为此,实现廉价且可大量制造的生物电极的持续工作仍然是主要挑战之一。在当前的工作中,通过利用定制的和新颖的3D打印(3DP)复合石墨烯/ PLA细丝创建了此类生物电极(生物阳极和生物阴极)。这种简单的方法经济高效,快速,消除了任何进一步修改和后期处理的要求,并适合在短时间内印刷最终产品。为了增加表面积并优化电化学感应,用二甲基甲酰胺(DMF)溶液处理3DP石墨烯/ PLA电极,然后固定GOx和漆酶,分别实现生物阳极和生物阴极。分别通过扫描电子显微镜(SEM)和能量分散光谱(EDS)对3DP,3DP / DMF处理和酶修饰的电极进行了形态和元素组成研究。使用线性扫描伏安法(LSV),循环伏安法(CV)和开路电势(OCP)评估了制备的生物电极的电化学和极化性能。在40 mM葡萄糖浓度下,生物阳极在0.5 V时的最大电流密度为1.41 mA / cm2,在生物阴极中则为0.42 V时的最大电流密度为0.216 mA / cm2。为了提供概念证明,将制备的生物电极组装在流体池中并研究其极化性能。总的来说,所制造的3DP生物电极表现出良好的稳定性,并具有相当好的生物催化活性保留。所制造的3DP复合石墨烯/ PLA电极是有前途的EBFC生物电极材料,在许多微流控水平上都是基于电化学的生物化学传感应用。