Background

Due to their sessile life style, plant survival is dependent on the ability to build up fast and highly adapted responses to environmental stresses by modulating defense response and organ growth. The phytohormone jasmonate plays an essential role in regulating these plant responses to stress.

Results

To assess variation of plant growth responses and identify genetic determinants associated to JA treatment, we conducted a genome-wide association study (GWAS) using an original panel of Vietnamese rice accessions. The phenotyping results showed a high natural genetic variability of the 155 tested rice accessions in response to JA for shoot and root growth. The level of growth inhibition by JA is different according to the rice varieties tested. We conducted genome-wide association study and identified 28 significant associations for root length (RTL), shoot length (SHL), root weight (RTW), shoot weight (SHW) and total weight (TTW) in response to JA treatment. Three common QTLs were found for RTL, RTW and SHL. Among a list of 560 candidate genes found to co-locate with the QTLs, a transcriptome analysis from public database for the JA response allows us to identify 232 regulated genes including several JA-responsive transcription factors known to play a role in stress response.

Conclusion

Our genome-wide association study shows that common and specific genetic elements are associated with inhibition of shoot and root growth under JA treatment suggesting the involvement of a complex JA-dependent genetic control of rice growth inhibition at the whole plant level. Besides, numerous candidate genes associated to stress and JA response are co-located with the association loci, providing useful information for future studies on genetics and breeding to optimize the growth-defense trade-off in rice.

中文翻译：

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利用全基因组关联研究揭示茉莉花酸酯参与水稻芽和根生长调控的遗传因素。

背景

由于它们的固执生活方式，植物的存活取决于能否通过调节防御反应和器官生长来建立对环境胁迫的快速且高度适应的反应。茉莉酮酸植物激素在调节这些植物对胁迫的反应中起重要作用。

结果

为了评估植物生长反应的变化并确定与JA处理相关的遗传决定因素，我们使用越南稻种的原始选材进行了全基因组关联研究（GWAS）。表型分析结果表明，受试的155个水稻品种对JA对芽和根生长的反应具有很高的自然遗传变异性。根据所测试的水稻品种，JA抑制生长的水平有所不同。我们进行了全基因组关联研究，并确定了响应JA处理的根长（RTL），枝长（SHL），根重（RTW），枝重（SHW）和总重（TTW）的28个显着关联。发现了RTL，RTW和SHL的三个常见QTL。在发现与QTL共同定位的560个候选基因中，

结论

我们的全基因组关联研究表明，在JA处理下，共同的和特定的遗传元素与芽和根生长的抑制有关，这表明在整个植物水平上涉及水稻生长抑制的复杂的JA依赖性遗传控制。此外，与胁迫和JA应答相关的许多候选基因与该关联基因位于同一位置，为将来的遗传和育种研究提供有用的信息，以优化水稻的生长防御权衡。