High-resolution 13C NMR spectroscopy has been used to analyze the positional distribution of fatty acids in model triacylglycerols. A novel method for quantitative determination of the positional distribution of unsaturated chains in triacylglycerols simultaneously with the ratio of saturated/unsaturated acyl chains has been proposed, utilizing the chemical shift differences of the aliphatic atoms C4, C5, and C6. The use of HSQC-TOCSY spectra allows unequivocal proof of the position of the unsaturated chain as well as complete assignment of the 13C NMR signals in tripalmitin.

中文翻译：

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定量测定三酰基甘油和相关化合物中酰基位置分布的另一种NMR方法。

高分辨率13C NMR光谱已用于分析模型三酰甘油中脂肪酸的位置分布。提出了一种利用脂肪族原子C4，C5和C6的化学位移差异，同时测定三酰基甘油中不饱和链的位置分布以及饱和/不饱和酰基链比率的新颖方法。HSQC-TOCSY光谱的使用可以明确证明不饱和链的位置，以及在Trimalmitin中完成13C NMR信号的完全分配。